GATA-1 Antibody (4F5)

Novus Biologicals | Catalog # NBP1-47492

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Baboon

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4F5
View all GATA-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GATA-1 expressed in E. Coli.

Reactivity Notes

Papio anubis reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-1 Antibody (4F5)

Western Blot: GATA-1 Antibody (4F5)BSA Free [NBP1-47492]

Western Blot: GATA-1 Antibody (4F5)BSA Free [NBP1-47492]

Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody (4F5) - BSA Free [NBP1-47492]

Immunocytochemistry/ Immunofluorescence: GATA-1 Antibody (4F5) - BSA Free [NBP1-47492]

Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GATA-1 Antibody (4F5) - BSA Free [NBP1-47492]

Immunohistochemistry: GATA-1 Antibody (4F5) - BSA Free [NBP1-47492]

Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.
Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) - BSA Free [NBP1-47492]

Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) - BSA Free [NBP1-47492]

Immunohistochemistry-Paraffin: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of pancreatic cancer, using GATA1 mouse mAb with DAB staining.

Applications for GATA-1 Antibody (4F5)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
Use in Chip reported in scientific literature (PMID:32763160)

Reviewed Applications

Read 1 review rated 4 using NBP1-47492 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATA-1

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Alternate Names

GATA1

Gene Symbol

GATA1

Additional GATA-1 Products

Product Documents for GATA-1 Antibody (4F5)

Certificate of Analysis

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Product Specific Notices for GATA-1 Antibody (4F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GATA-1 Antibody (4F5)

Customer Reviews for GATA-1 Antibody (4F5) (1)

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Customer Images

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  • GATA-1 Antibody (4F5)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: fetal liver
    Species: Papio anubis
    Verified Customer | Posted 02/26/2019
    GATA-1 Staining of Fetal Baboon Liver 400X
    IHC paraffin, species Papio anubis (Olive Baboon), fetal liver Antigen retrieval 10mM sodium citrate, pH 6.0 Image-iT signal enhancer Streptavidin/Biotin blocking kit Primary Ab: 1:500 dilution Second Ab (biotinylated): 1:200 Streptavidin-AlexaFluor488: 1:500 (green) Quench autofluorescence with 1% Sudan Black in 70% methanol Counterstain nuclei with propidium iodide (red)
    GATA-1 Antibody (4F5) NBP1-47492

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Protocols

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