GATA-3 Antibody (GATA3/2442)
Novus Biologicals | Catalog # NBP3-07661
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # GATA3/2442
Loading...
Product Specifications
Immunogen
Recombinant fragment of human GATA-3 protein (around aa 357-436) (exact sequence is proprietary) (Uniprot: P23771)
Localization
Nuclear
Marker
Breast and Urothelial Marker
Specificity
GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas.'Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08401)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for GATA-3 Antibody (GATA3/2442)
![Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-GATA3-2442-Immunohistochemistry-Paraffin-NBP3-07661-img0002.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).![Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]](https://resources.rndsystems.com/images/products/GATA-3-Antibody-GATA3-2442-Immunohistochemistry-Paraffin-NBP3-07661-img0001.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]")
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661]
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/2442) [NBP3-07661] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).Applications for GATA-3 Antibody (GATA3/2442)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: GATA-3
Additional GATA-3 Products
Product Documents for GATA-3 Antibody (GATA3/2442)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GATA-3 Antibody (GATA3/2442)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for GATA-3 Antibody (GATA3/2442)
There are currently no reviews for this product. Be the first to review GATA-3 Antibody (GATA3/2442) and earn rewards!
Have you used GATA-3 Antibody (GATA3/2442)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
