GATA-3 Antibody (OTI8H4)

Novus Biologicals | Catalog # NBP2-45810

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8H4
View all GATA-3 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-3 Antibody (OTI8H4)

Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis (35 ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody at 1:200.
Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Mouse lung tissue section stained with GATA-3 antibody. IHC-P image submitted by a verified customer review.
Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Flow cytometric Analysis of penetrated MCF-7 cells, compared to a nonspecific negative control antibody, (Blue). The right is HUVEC cells as negtive control (1:100).
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human colon tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human Kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human endometrium tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human bladder tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.
Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810]

Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human lymphoma tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.

Applications for GATA-3 Antibody (OTI8H4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:200

Western Blot

1:200

Reviewed Applications

Read 1 review rated 5 using NBP2-45810 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (OTI8H4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GATA-3 Antibody (OTI8H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GATA-3 Antibody (OTI8H4)

Customer Reviews for GATA-3 Antibody (OTI8H4) (1)

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Customer Images

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  • GATA-3 Antibody (OTI8H4)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 12/08/2021
    Lung tissue
    4% formaldehyde fixed paraffin sections
    GATA-3 Antibody (OTI8H4) NBP2-45810

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Protocols

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FAQs

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