GDF-15 Antibody - BSA Free

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050]

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human prostate shows moderate cytoplasmic positivity in a small subset of glandular cells.

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050]

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050]

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human placenta shows very strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050]

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.

Immunohistochemistry: GDF-15 Antibody - BSA Free [NBP1-81050] -

GDF‐15 expression is modulated by ROS‐induced p53 activation. (A) Relative release of GDF‐15 at 7 days and 14 days after ex vivo cartilage trauma of untreated or NAC‐treated explants. (B) Gene expression of GDF15 and (C, D) detection of intracellular ROS levels by DCFDA assay in isolated hAC upon H2O2 exposure for 48 h. (E) Release of GDF‐15 by H2O2‐treated hAC with or without treatment of NAC. (F) Representative images of IF staining of p53 in H2O2‐treated hAC. (G) Correlation analysis between CTCF values of p53 staining and GDF‐15 concentrations of unstimulated or H2O2‐treated hAC. (H) Quantification of posttraumatic p53 activation (detected by means of IHC) and effects of antioxidative therapy by NAC at 7 days after ex vivo cartilage trauma. Gene expression analysis of GDF‐15 in hAC after stimulation with (I) the MDM2 antagonist (p53 inducing) Nutlin‐3a and (J) the inhibitor of p53 transcription activity, Pifithrin‐ alpha or Pifithrin‐μ. (K) Representative images of IHC staining against p53 and GDF‐15 in human cartilage and (L) corresponding correlation analysis. The white bars in (C) and (F) represent 100 um. The black bars in (K) represent 200 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41287825), licensed under a CC-BY license. Not internally tested by Novus Biologicals.