Gephyrin/GPHN Antibody (OTI3B6)

Novus Biologicals | Catalog # NBP2-03449

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3B6
View all Gephyrin/GPHN Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GPHN (NP_065857) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

83.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gephyrin/GPHN Antibody (OTI3B6)

Western Blot: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Western Blot: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Gephyrin monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunocytochemistry/ Immunofluorescence: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunocytochemistry/Immunofluorescence: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Gephyrin.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human prostate tissue using anti-Gephyrin mouse monoclonal antibody.
Flow Cytometry: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Flow Cytometry: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Gephyrin antibody, and then analyzed by flow cytometry.
Western Blot: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Western Blot: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Western Blot: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Gephyrin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Gephyrin.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Gephyrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Gephyrin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Immunohistochemistry-Paraffin: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Staining of paraffin-embedded Human liver tissue using anti-Gephyrin mouse monoclonal antibody.
Flow Cytometry: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Flow Cytometry: Gephyrin/GPHN Antibody (OTI3B6) [NBP2-03449]

Flow Cytometry: Gephyrin/GPHN Antibody (3B6) [NBP2-03449] - Analysis of Jurkat cells, using anti-Gephyrin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Gephyrin/GPHN Antibody (OTI3B6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 1 review rated 5 using NBP2-03449 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gephyrin/GPHN

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq].

Alternate Names

GEPH, GPH, GPHN, GPHRYN

Entrez Gene IDs

10243 (Human)

Gene Symbol

GPHN

Additional Gephyrin/GPHN Products

Product Documents for Gephyrin/GPHN Antibody (OTI3B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Gephyrin/GPHN Antibody (OTI3B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors
Neuronal Markers

Customer Reviews for Gephyrin/GPHN Antibody (OTI3B6) (1)

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  • Name: Fugui Li
    Application: Immunofluorescence
    Sample Tested: mouse brain sections
    Species: Mouse
    Verified Customer | Posted 10/06/2014

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