GFI-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-27173

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Chicken

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GFI-1 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acid 280-330 of human Zinc finger protein Gfi-1 was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GFI-1 Antibody - BSA Free

Western Blot: GFI-1 Antibody [NBP2-27173]

Western Blot: GFI-1 Antibody [NBP2-27173]

Western Blot: GFI-1 Antibody [NBP2-27173] - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173]

Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173]

Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173] - Analysis of Gfi-1 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.

Applications for GFI-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

6-8ug/ml~

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFI-1

Gfi-1 is a nuclear zinc finger (ZF) transcriptional repressor that plays an important role in hematopoiesis and inner ear development, and has been implicated in lymphomagenesis. The Gfi1 protein expression is regulated by the ubiquitin-proteasome system. It is reported that ubiquitin ligase Triad1 interacts with the DNA-binding domain of Gfi-1. Gfi-1 represses transcription by directly binding to the consensus DNA sequence in the promoters of its target genes. Deficiency of Gfi-1 causes neutropenia and an accumulation of granulomonocytic precursor cells that is reminiscent of a myelodysplastic syndrome in mice.

Long Name

Growth Factor Independent 1

Alternate Names

GFI1, Pal-1, ZNF163

Gene Symbol

GFI1

Additional GFI-1 Products

Product Documents for GFI-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GFI-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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