GFP Antibody (859) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43575

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Human, Insect - Drosophila, Jellyfish

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 859

Format

Azide and BSA Free
View all GFP Primary Antibodies »

Product Specifications

Immunogen

Full length GFP recombinant protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GFP Antibody (859) - Azide and BSA Free

ELISA: GFP Antibody (859) [NBP2-43575]

ELISA: GFP Antibody (859) [NBP2-43575]

ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)
Immunoprecipitation: GFP Antibody (859) [NBP2-43575]

Immunoprecipitation: GFP Antibody (859) [NBP2-43575]

Immunoprecipitation: GFP Antibody (859) [NBP2-43575] - Analysis of 400 ug cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ug of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.
GFP Antibody (859) - Azide and BSA Free

Western Blot: GFP Antibody (859) [NBP2-43575] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [GT859] (NBP2-43575) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
GFP Antibody (859) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GFP Antibody (859) [NBP2-43575] -

GFP antibody [GT859] detects GFP protein by immunofluorescent analysis.
Sample: GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: GFP stained by GFP antibody [GT859] (NBP2-43575) diluted at 1:2000.
Blue: Hoechst 33342 staining.
GFP Antibody (859) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GFP Antibody (859) [NBP2-43575] -

GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red:.GFP-recombinant protein stained by GFP-tag antibody (NBP2-43575) diluted at 1:2000. Blue: Hoechst 33342 staining.

Applications for GFP Antibody (859) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:2000

Immunohistochemistry

10 - 1:500

Immunohistochemistry Whole-Mount

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000
Application Notes
Use in In vivo reported in scientific literature (PMID:34246771).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

Additional GFP Products

Product Documents for GFP Antibody (859) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GFP Antibody (859) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GFP Antibody (859) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GFP Antibody (859) - Azide and BSA Free

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  • Q: We are looking for a rabbit anti-GFP antibody as a primary antibody for immunofluorescence and WB. I've seen that you carry several anti-GFP antibodies and I would like to ask which one you would recommend. The one with Cat.No NB600-308 has been used in a lot of publications and I reckon it is a solid antibody. Is this our best choice, or is there a better alternative?

    A: NB600-308 is an excellent GFP antibody and I think it would work very well for you. It has been reviewed and published with which usually makes our customer feel much more confident, seeing as it worked in other people's hand.

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