GilZ Antibody (3A5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001831-M01

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A5

Format

Azide and BSA Free
View all GilZ Primary Antibodies »

Product Specifications

Immunogen

TSC22D3 (NP_932174, 1 a.a. ~ 97 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQSKLDCRSPVGLDCCNCCLDLAHRSGLQRGSSGENNNPGSPTVSNFRQLQEKLVFENLNTDKLNSIMRQDSLEPVLRDPCYLINEGICNRNIDQT

Specificity

TSC22D3 - TSC22 domain family, member 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GilZ Antibody (3A5) - Azide and BSA Free

Western Blot: GilZ Antibody (3A5) [H00001831-M01]

Western Blot: GilZ Antibody (3A5) [H00001831-M01]

Western Blot: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody (M01), clone 3A5.Lane 1: TSC22D3 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: GilZ Antibody (3A5) [H00001831-M01]

Immunocytochemistry/ Immunofluorescence: GilZ Antibody (3A5) [H00001831-M01]

Immunocytochemistry/Immunofluorescence: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01]

Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01]

Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Human lymph node. Antigen retrieval in citrate buffer at 95C for 30 min. Image from verified customer review.
Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01]

Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01]

Immunohistochemistry-Paraffin: GilZ Antibody (3A5) [H00001831-M01] - Analysis of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Immunoprecipitation: GilZ Antibody (3A5) [H00001831-M01]

Immunoprecipitation: GilZ Antibody (3A5) [H00001831-M01]

Immunoprecipitation: GilZ Antibody (3A5) [H00001831-M01] - Analysis of TSC22D3 transfected lysate using anti-TSC22D3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TSC22D3 MaxPab rabbit polyclonal antibody.
ELISA: GilZ Antibody (3A5) [H00001831-M01]

ELISA: GilZ Antibody (3A5) [H00001831-M01]

ELISA: GilZ Antibody (3A5) [H00001831-M01] - Detection limit for recombinant GST tagged TSC22D3 is approximately 0.03ng/ml as a capture antibody.

Applications for GilZ Antibody (3A5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

3 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, IF and ELISA.

Reviewed Applications

Read 1 review rated 4 using H00001831-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GilZ

The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

Delta sleep-inducing peptide immunoreactor, DSIP-immunoreactive leucine zipper protein, DSIP-immunoreactive peptide, GILZDKFZp313A1123, Glucocorticoid-induced leucine zipper protein, Protein DIP, TSC22 domain family protein 3, TSC22 domain family, member 3, TSC-22 related protein, TSC-22-like protein, TSC-22-related protein, TSC-22Rimmunoreactor

Entrez Gene IDs

1831 (Human)

Gene Symbol

TSC22D3

OMIM

300506 (Human)

UniProt

NP_932174 (Human)

Additional GilZ Products

Product Documents for GilZ Antibody (3A5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GilZ Antibody (3A5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GilZ Antibody (3A5) - Azide and BSA Free

Customer Reviews for GilZ Antibody (3A5) - Azide and BSA Free (1)

4 out of 5
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  • GilZ Antibody (3A5)
    Name: Santhosh Sivajothi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lymph node
    Species: Human
    Verified Customer | Posted 09/25/2018
    Heat mediated antigen retrieval performed in citrate buffer (30 min @ 95C)
    GilZ Antibody (3A5) - Azide and BSA Free H00001831-M01

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Protocols

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FAQs

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