GLI-1 Antibody - BSA Free

Immunohistochemistry: GLI-1 Antibody - BSA Free [NBP1-78259]

GLI-1-Antibody-Immunohistochemistry-NBP1-78259-img0010.jpg

Immunohistochemistry: GLI-1 Antibody - BSA Free [NBP1-78259]

Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 in mouse brain using DAB with hematoxylin counterstain.

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody - BSA Free [NBP1-78259] -

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody - BSA Free [NBP1-78259] - In vivo GLI1 expression after intratumoral administration of NVP-LDE225.LOX OMVI human melanoma cells were injected s.c into both flanks as mentioned above. Tumors were treated intratumorally on daily basis with vehicle (A & B) or NVP-LDE225 (C & D). Immunofluorescent microscopy of GLI1was performed on isolated tumor tissues. GLI1 staining was performed by overnight incubation of sections at 4°C with rabbit anti-human polyclonal Ab (B & D, NBP1-78259, Novus Biologicals, Littleton, CO) or isotype control (A & C) followed by an 1 hr-incubation with Alexa Fluor® 488 Donkey IgG, anti-rabbit (A21206, Invitrogen, Carlsbad, CA) at RT (green). Counterstaining of nuclei was performed with propidium iodide (red). Pictures were taken on a confocal laser-scanning microscope system (LSM 410; Zeiss). Yellow color corresponds to double positive (anti-GLI1 & propidium iodide) nuclear staining. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23935925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GLI-1 Antibody - BSA Free [NBP1-78259] -

Hedgehog signaling activation fails to promote regeneration under inflammatory conditions. A) GLI1 and CCND1 protein expression in dermal fibroblasts treated with SAG or the control vector for 2 days. B) Cell proliferation fold change in dermal fibroblasts at 24, 48, and 72 h after co‐culture within RAW 264.7 cells supernatant with (sti‐RAW) or without LPS stimulation and supplemented with a vector or 1 um SAG, as determined using a Cell Counting Kit‐8 assay. Data presented as the mean +/- SD, n = 5 biologically independent samples. P‐values were calculated using ANOVA with Tukey's multiple comparisons test. *P < 0.05. NS, not significant, P > 0.05. C) Immunofluorescence staining for Ki67 (red) and visualization of EdU (green) in dermal fibroblasts after co‐culture within the supernatant of sti‐RAW 264.7 cells or RAW 264.7 cells without LPS stimulation supplemented with vector or 1 um SAG for 48 h. D) Representative images and E) quantification of the results of the in vitro scratch assay of dermal fibroblasts co‐cultured within the supernatant of sti‐RAW 264.7 cells or RAW 264.7 cells without LPS stimulation supplemented with vector or 1 um SAG for 48 h. Dotted lines indicate the initial scratch edges. Data are presented as the mean +/- SD, n = 4. P‐values were calculated using ANOVA with Tukey's multiple comparisons test. *P < 0.05. NS, not significant, P > 0.05. F) Representative images and G) quantification of tube formation in HUVECs co‐cultured within the supernatant of sti‐RAW 264.7 cells or RAW 264.7 cells without LPS stimulation supplemented with vector or 1 um SAG for 2 h. Data are presented as the mean +/- SD, n = 5. P‐values were calculated using ANOVA with Tukey's multiple comparisons test, *P < 0.05. NS, not significant, P > 0.05. Scale bar, 200 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39413023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.