Glu-Glu Epitope Tag Antibody - BSA Free

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• Glu-Glu Epitope Tag Antibody - BSA Free

  Name: Kalynn Alexander

  Application: Flow Cytometry

  Sample Tested: NMuMG mouse mammary gland epithelial cell line and Mouse blood

  Species: Mouse

  Verified Customer | Posted 08/24/2023

  Histogram results from flow cytometry on mouse polyomavirus infected and non-infected NMuMg cells and mouse blood. No difference is seen between the infected and non-infected groups.

  Based on the principle that the Glu-Glu epitope tag is derived from the sequence for mouse polyomavirus Middle T we hypothesized that the anti-Glu-Glu tag might be able to stain middle T antigen in mouse polyomavirus infected cells. We grew two groups of mice NMuMg cells, infecting one with A2 strain mouse polyomavirus for 24 hours and leaving one culture uninfected. The cells were lifted from the plates and prepped for flow cytometry including permeabilizing and staining with the anti-Glu-Glu Tag. The antibody stained a majority of the cells in both groups as positive, demonstrating that the antibody is not specific. Unstained cells were included for comparison. The same experiment was repeated with mouse blood from both infected and non-infected animals producing the same results. In conclusion the Glu-Glu tag antibody does not specifically recognize middle T in cells infected by mouse polyomavirus by flow cytometry. This was an unvalidated application.

  
  Bio-Techne Response

  This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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