Q: Could you help this customer find a monoclonal antibody specific to rat's Glut2 protein to be used in IHC. I looked on our website and found one specific to Human's Glut2. Does this one cross react with Rat as well?

A: I have found the following antibody that would be suitable for you: NBP1-52560 I have included the peptide sequence below for the customer so that they can verify the homology with Rat. It is predicted and guaranteed to work in rat samples based on the sequence homology. If this antibody does not perform as expected you may receive a refund or replacement.

Glut2 Antibody

Novus Biologicals | Catalog # NBP1-52560

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bat (93%), Equine (93%), Monkey (93%), Porcine (93%), Primate (93%), Rabbit (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Glut2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide C-RKEREEASSEQKVS from an internal region of human Glut2 (NP_000331.1).

Epitope

C-RKEREEASSEQKVS from an internal region

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (93%), Rat (86%), Sheep (86%), Canine (86%), Bovine (86%).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glut2 Antibody

Immunohistochemistry-Paraffin: Glut2 Antibody [NBP1-52560]

Immunohistochemistry-Paraffin: Glut2 Antibody [NBP1-52560] - Analysis of anti-GLUT2 antibody with human liver at concentration 2.5 ug/ml.

Applications for Glut2 Antibody

Application

Recommended Usage

Flow Cytometry

10 ug/ml

Immunofluorescence

10 ug/ml

Immunohistochemistry

2-6 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

1:8000

Application Notes

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut2

Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as Glut 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers. The majority of differences in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are asymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood testis barriers.

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Entrez Gene IDs

6514 (Human)

Gene Symbol

SLC2A2

UniProt

P11168

Additional Glut2 Products

Product Documents for Glut2 Antibody

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Certificate of Analysis

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Product Specific Notices for Glut2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes

Glucose Homeostasis

Metabolism

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