Glut3 Antibody

Novus Biologicals | Catalog # NBP3-12237

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all Glut3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 400-493 on mouse SLC2A3 protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Glut3 Antibody

Western Blot: Glut3 Antibody [NBP3-12237]

Western Blot: Glut3 Antibody [NBP3-12237]

Western Blot: Glut3 Antibody [NBP3-12237] - WB of NBP3-12237 with rat brain. 1:500 antibody dilution in DiluObuffer. Apparent MW is 45-47 kDa.
Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237]

Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237]

Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237] - Rat testis. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstrain. 40X magnification. FFPE section.
Western Blot: Glut3 Antibody [NBP3-12237]

Western Blot: Glut3 Antibody [NBP3-12237]

Western Blot: Glut3 Antibody [NBP3-12237] - Cortical extracts (10 ug total protien/lane) on 10% gel. Primary antibody glucose transporter type 3 diluted 1:2000 in Dilobuffer incubation in primary anitbody 120 minutes at RT; washes 3x10 minutes in TBST secondary antibody alkaline phosphatase conjugated goat anti-rabbit IgG (1:5000)
Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237]

Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237]

Immunohistochemistry-Paraffin: Glut3 Antibody [NBP3-12237] - Mouse Spleen-NBP3-12237. 1:100 dilution in IHC blocking buffer. DAB (brown) straining and Hematoxylin QS (blue) counterstrain. 40X magnification. FFPE section

Applications for Glut3 Antibody

Application
Recommended Usage

ELISA

1:25000

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:8000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Glut3

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Gene Symbol

SLC2A3

Additional Glut3 Products

Product Documents for Glut3 Antibody

Certificate of Analysis

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Product Specific Notices for Glut3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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