Glut4 Antibody

Novus Biologicals | Catalog # NBP1-49840

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glut4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TELEYLGPDEND corresponding to C-Terminus according to NP_001033.1.

Reactivity Notes

Expected from sequence similarity: Bovine, Porcine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glut4 Antibody

Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840]

Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840]

Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840]

Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Glut4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3-1 ug/ml
Application Notes
Western blot: Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997 Mar 14;272(11):7085-92.; PMID: 9054401). Primary incubation was 1 hour
IHC: Paraffin embedded Human Skeletal Muscle.

Reviewed Applications

Read 1 review rated 4 using NBP1-49840 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut4

Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient. Insulin resistant subjects with and without diabetes show abnormal accumulation of GLUT4 in a dense membrane compartment in muscle and adipocytes from which insulin is unable to recruit GLUT4 to the cell surface.

Alternate Names

SLC2A4

Entrez Gene IDs

6517 (Human); 20528 (Mouse); 25139 (Rat)

Gene Symbol

SLC2A4

UniProt

P14672

Additional Glut4 Products

Product Documents for Glut4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Glut4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Glut4 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Glut4 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Eric England
    Application: Western Blot
    Sample Tested: Whole Skeletal Muscle Lysate
    Species: Other
    Verified Customer | Posted 09/30/2014
    Glut4 Antibody NBP1-49840

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies