Glutamate Dehydrogenase Antibody - BSA Free

Novus Biologicals | Catalog # NB600-853

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Format

BSA Free
View all Glutamate Dehydrogenase 1/GLUD1 Primary Antibodies »

Product Specifications

Immunogen

This Glutamate Dehydrogenase Antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver. (Uniprot: P00366)

Reactivity Notes

BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for Glutamate Dehydrogenase from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon.
Human reactivity reported in scientific literature (PMID: 27623078).

Localization

Mitochondrial matrix

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glutamate Dehydrogenase [Bovine Liver]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glutamate Dehydrogenase Antibody - BSA Free

Western Blot: Glutamate Dehydrogenase Antibody [NB600-853]

Western Blot: Glutamate Dehydrogenase Antibody [NB600-853]

Western Blot: Glutamate Dehydrogenase Antibody [NB600-853] - Western blot analysis is shown using Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
N/A: Glutamate Dehydrogenase Antibody [NB600-853]

Applications for Glutamate Dehydrogenase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:4000-1:16000

Immunoprecipitation

1:100

Western Blot

1:1000-1:3000
Application Notes
This antibody has been tested by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract. Anti-Glutamate Dehydrogenase Antibody is suitable for use in ELISA.

Use in Immunohistochemistry reported in scientific literature (PMID 27623078).

Reviewed Applications

Read 1 review rated 4 using NB600-853 in the following applications:

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 1/GLUD1

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Alternate Names

GDH, GDH1

Entrez Gene IDs

281785 (Bovine)

Gene Symbol

GLUD1

UniProt

P00366 (Bovine)

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glutamate Dehydrogenase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutamate Dehydrogenase Antibody - BSA Free

Customer Reviews for Glutamate Dehydrogenase Antibody - BSA Free (1)

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  • Glutamate Dehydrogenase Antibody
    Name: Paula Chen
    Application: Western Blot
    Sample Tested: Porcine embryo
    Species: Pig
    Verified Customer | Posted 01/09/2019
    Analysis of glutamate dehydrogenase expression in day 6 porcine blastocysts after culture for 6 days in 0, 1, 3.75, or 10 mM glutamine.
    A 4-20% gradient gel was used to separate proteins from pools of 40 blastocysts before transfer to PVDF membranes. The membrane was incubated in 1:1000 anti-glutamate dehydrogenase primary overnight at 4 degrees Celsius. The next day, membranes were incubated in 1:10000 anti-rabbit IgG HRP secondary for 1 hour.
    Glutamate Dehydrogenase Antibody - BSA Free NB600-853

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