Glutamate Dehydrogenase Antibody

Novus Biologicals | Catalog # NBP2-16679

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Cited:

Human, Mouse

Predicted:

Bovine (98%), Porcine (99%), Sheep (99%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Glutamate Dehydrogenase 1/GLUD1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%).

Localization

Mitochondrion matrix

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamate Dehydrogenase Antibody

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679]

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679]

Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP2-16679-img0012.jpg
Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679]

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679]

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Human breast cancer, using Glutamate Dehydrogenase antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Mouse kidney. Glutamate Dehydrogenase stained by Glutamate Dehydrogenase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Glutamate Dehydrogenase Antibody

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] -

Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] -Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1+ GLUD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Glutamate Dehydrogenase Antibody

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] -

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Glutamate Dehydrogenase Antibody

Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] -

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200.
Blue: Hoechst 33342 staining.
Glutamate Dehydrogenase Antibody

Immunocytochemistry/ Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] -

GLUD1+ GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Glutamate Dehydrogenase Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 1/GLUD1

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]

Alternate Names

GDH, GDH1

Gene Symbol

GLUD1

UniProt

P00367 (Human)

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Antibody

Certificate of Analysis

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Product Specific Notices for Glutamate Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Glutamate Dehydrogenase Antibody

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Protocols

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