Glutathione Peroxidase 2/GPX2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-32002
![Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]](https://resources.rndsystems.com/images/products/Glutathione-Peroxidase-2-GPX2-Antibody-Western-Blot-NBP1-32002-img0019.jpg "Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]")
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Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutathione Peroxidase 2/GPX2. The exact sequence is proprietary.
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Glutathione Peroxidase 2/GPX2 Antibody - BSA Free
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Glutathione Peroxidase 2/GPX2 antibody detects GPX2 protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and blotted with Glutathione Peroxidase 2/GPX2 antibody diluted by 1:500![Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]](https://resources.rndsystems.com/images/products/Glutathione-Peroxidase-2-GPX2-Antibody-Immunohistochemistry-Paraffin-NBP1-32002-img0008.jpg "Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]")
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Human gastric cancer, using antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min![Immunoprecipitation: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]](https://resources.rndsystems.com/images/products/Glutathione-Peroxidase-2-GPX2-Antibody-Immunoprecipitation-NBP1-32002-img0015.jpg "Immunoprecipitation: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]")
Immunoprecipitation: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002]
Immunoprecipitation: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 ug of GPX2 antibody [C1C3]. Western blot analysis was performed using GPX2 antibody [C1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Mouse stomach.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -Rat duodenum.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Mouse stomach.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ICC/IF-Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
ICC/IF-Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Mouse intestine.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Rat liver.GPX2 stained by GPX2 antibody [C1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry-Paraffin: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Glutathione Peroxidase 2/GPX2 antibody [C1C3] detects Glutathione Peroxidase 2/GPX2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse esophagus.
Glutathione Peroxidase 2/GPX2 stained by Glutathione Peroxidase 2/GPX2 antibody [C1C3] (NBP1-32002) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Immunohistochemistry: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Immunohistochemical analysis of paraffin-embedded mouse muscle, using Glutathione Peroxidase 2/GPX2(NBP1-32002) antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione Peroxidase 2/GPX2 antibody [C1C3] (NBP1-32002) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NBP1-32002] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione Peroxidase 2/GPX2 antibody [C1C3] (NBP1-32002) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for Glutathione Peroxidase 2/GPX2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Glutathione Peroxidase 2/GPX2
Alternate Names
GIGPX, GPRP, GPX2
Gene Symbol
GPX2
Additional Glutathione Peroxidase 2/GPX2 Products
Product Documents for Glutathione Peroxidase 2/GPX2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Glutathione Peroxidase 2/GPX2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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