Glycogen Synthase [p Ser641] Antibody - BSA Free

Novus Biologicals | Catalog # NB600-611

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Signal Stimulation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Glycogen Synthase Primary Antibodies »

Product Specifications

Immunogen

Human Muscle Glycogen Synthase phospho peptide corresponding to a S641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: P13807)

Reactivity Notes

Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined

Modification

p Ser641

Specificity

Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS641 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glycogen Synthase [p Ser641] Antibody - BSA Free

Western Blot: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Affinity Purified Glycogen Synthase [p Ser641] antibody.Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'.Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'.Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'.Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4nM for 45'. Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'. Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'. Load: 12uL. Primary Antibody: pS641 at 1:1000. Secondary Antibody: HRP conjugated Gt-a-Rabbit IgG at 1:5,000 dilution preceded color development using Amersham's substrate system. Other detection methods will yield similar results.
Immunohistochemistry: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Immunohistochemistry with Glycogen Synthase [p Ser641] antibody.Tissue: Human Prostate.Fixation: formalin-fixed, paraffin-embedded tissue.Antigen retrieval: heat-induced.Primary antibody: 5 ug/ml.Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Signal Stimulation: Glycogen Synthase [p Ser641] Antibody [NB600-611]

Signal Stimulation: Glycogen Synthase [p Ser641] Antibody [NB600-611]

Signal Stimulation: Glycogen Synthase [p Ser641] Antibody [NB600-611] - Diagram of glycogen synthase as a component of insulin signal transduction pathways.

Applications for Glycogen Synthase [p Ser641] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:60000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Western Blot

1:1000-1:10000
Application Notes
This This product was tested by immunoblotting, immunohistochemistry, and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glycogen Synthase

Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at Ser 640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.

Alternate Names

EC 2.4.1.11, glycogen [starch] synthase, muscle, glycogen synthase 1 (muscle), GYSGSY

Entrez Gene IDs

2997 (Human); 14936 (Mouse)

Gene Symbol

GYS1

UniProt

P13807 (Human)

Additional Glycogen Synthase Products

Product Documents for Glycogen Synthase [p Ser641] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glycogen Synthase [p Ser641] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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