Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80755

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Glo1a

Format

Azide and BSA Free
View all Glyoxalase I Primary Antibodies »

Product Specifications

Immunogen

Full length human GLO1 protein [Swiss-Prot #Q04760].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

Western Blot: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Western Blot: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Western Blot: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - HeLa whole cell extracts. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755]

Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755]

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University. Image from the standard format of this antibody.
Western Blot: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Western Blot: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Western Blot: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. This image was submitted via customer Review. Image from the standard format of this antibody.
Simple Western: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Simple Western: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Simple Western: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from the standard format of this antibody.
Simple Western: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Simple Western: Glyoxalase I Antibody (Glo1a)Azide and BSA Free [NBP2-80755]

Simple Western: Glyoxalase I Antibody (Glo1a) - Azide and BSA Free [NBP2-80755] - Simple Western lane view shows a specific band detected for GLO1 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa). Image from the standard format of this antibody.

Applications for Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.15 ng / 1 ug protein

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunoprecipitation

reported in scientific literature

Simple Western

1:50
Application Notes
In Western blot, a band is seen approx. 21 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:50. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glyoxalase I

Glyoxalase 1 (GLO1) is the main enzyme in glyoxalase system, a metabolic pathway responsible for detoxifying alpha-oxoaldehydes, particularly methylglyoxal; and is known for converting reactive dicarbonyls into non-toxic intermediates ultimately protecting living cells from producing and accumulating advanced glycation end products, AGEs. GLO1 has also been studied with reference to anxiety, and a murine model showed that this gene's overexpression increases anxiety-related behavior by reducing the GABAA receptor agonist MG. Moreover, an association analysis in a human population with anxiety disorder subtypes suggested a role for GLO1's implication in this phenotype. GLO1 plays an important role in tumour growth and partly in chemotherapy survival as evidenced by a number of studies suggesting that some tumours rely on GLO1 to increase their viability and resistance to multiple chemotherapeutic agents. Furthermore, genetic studies have implicated GLO1 polymorphisms in panic disorder and restless legs syndrome (RLS).

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Glyoxalase I Antibody (Glo1a) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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