GM-CSF Antibody (OTI8G5)

Novus Biologicals | Catalog # NBP2-46364

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8G5
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Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 18-144 of human CSF2(NP_000749) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GM-CSF Antibody (OTI8G5)

Western Blot: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Western Blot: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Western Blot: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GM-CSF.
Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) [NBP2-46364]

Immunohistochemistry: GM-CSF Antibody (8G5) [NBP2-46364] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for GM-CSF Antibody (OTI8G5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Application Notes
Use in IHC-P reported in scientific literature (PMID:32039403).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GM-CSF

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Gene Symbol

CSF2

Additional GM-CSF Products

Product Documents for GM-CSF Antibody (OTI8G5)

Certificate of Analysis

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Product Specific Notices for GM-CSF Antibody (OTI8G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GM-CSF Antibody (OTI8G5)

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Protocols

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