GMP Cloudz Human Treg Cell Expansion Kit
GMP Cloudz Human Treg Cell Expansion Kit Summary
Key Benefits
• > 250-fold expansion of human regulatory T (Treg) Cells from CD4+ T cells in 9 days
• Dissolvable CD3/28 microspheres enable Treg cell expansions
• Optimized for use with the ExCellerate™ Human T Cell Media
• Supports Treg cell expansion from CD4-positive cells
• Optimized for use with R&D Systems cytokines and media
Why Expand Treg Cells ex vivo Using GMP Cloudz Human Treg Cell Expansion Kit?
Treg cells are a suppressive subset of CD4+ T cells that function to antagonize immune responses. Treg cells have the capacity to prevent potentially damaging autoimmune and protective immune responses, so the number of Treg cells is a crucial determinant of the regulatory burden on the immune system. Treg cells prevent autoimmune disease, maintain immune homeostasis, and modulate immune responses during infection. The Cloudz Human Treg Expansion Kit is designed for the robust expansion of human regulatory T (Treg) cells from CD4+ T cells. The kit contains dissolvable microspheres that are functionalized with CD3 and CD28 antibodies.
Versatility of GMP Cloudz Human Treg Cell Expansion Kit
GMP Cloudz Human Treg Cell Expansion Kit is optimized for Treg cell expansion when used in combination with
Kit Contents
• 16 mL of GMP-Grade Treg Cloudz CD3/CD28
• 100 mL of GMP-Grade Cloudz 6X Release Buffer
• Each kit contains enough reagents to activate 1x108 CD4+ T Cells
Specifications
Product Datasheets
Data Example
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Fold Expansion of Treg Cells using the GMP Cloudz Human Treg Cell Expansion Kit Primary human CD4+ cells were activated with GMP-Grade Cloudz Treg CD3/CD28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Expansion of FoxP3+ Treg cells was evaluated using flow cytometry on Day 5 and Day 9 and fold expansion of was calculated relative to Day 0. Error bars reflect standard deviation across 3 donors.
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Fold Expansion of CD4+/CD25+/CD127- Treg Cells using the GMP Cloudz Human Treg Cell Expansion Kit Primary human CD4+ cells were activated with GMP-Grade Cloudz Treg CD3/CD28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Expansion of CD4+/CD25+/CD127- Treg cells was evaluated using flow cytometry on Day 5, 7 and 9 and fold expansion of was calculated relative to Day 0. Error bars reflect standard deviation across 3 donors.
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Increased FoxP3+ CD4 Cells following Treg Expansion. Flow cytometry data showing expression of the Treg cell marker, FoxP3, in CD4+ T cells on Day 0 (A, C) and Day 9 (B, D) following expansion using the GMP Cloudz Human Treg Expansion Kit. Nine days after expansion, the cells were fixed, permeabilized, and stained with CD4-FITC, CD25-APC, and FoxP3-PE antibodies. Cells in panels A and B were stained with FoxP3 and CD4. Cells in C and D were stained with FoxP3 and CD25.
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Viability greater than 90% after 9-day expansion Viability of Human Treg after expansion with GMP Cloudz Human Treg Cell Expansion Kit. Primary human CD4+ cells were activated with GMP-Grade Cloudz Treg CD3/28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Cell viability after expansion for 9 days was greater than 90%. Error bars reflect standard deviation across 3 donors.
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Fold expansion of Treg Cells with GMP Cloudz Human Treg Cell Expansion Kit compared to other suppliers Fold expansion of CD25+/CD4+/FoxP3+ Treg Cells is greater with GMP Cloudz Human Treg Cell Expansion Kit compared to other GMP-Grade Treg Cell Expansion suppliers.
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