GMP Cloudz Human Treg Cell Expansion Kit

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.

A: When in culture, the Cloudz particles are heterogeneous in size with an average diameter similar to that of a T cell.  

A: We do not perform this type of separation is house; we would only recommend the use of the Release Buffer provided in the kit. 

A: We have not tested the Cloudz Human Treg expansion kit, Catalog # CLD006, with mouse Treg cells.

A: The Cloudz microspheres are translucent, but can be seen as faint circles underneath the layer of cells when looking at the culture through a microscope. The same brightfield setting and microscope magnification that are useful for visualizing cells can be used to visualize the microspheres.

A: Yes. Cloudz microspheres sizes can be custom made to between 2-200 µm to match your desired application. For more information, check out our Cloudz landing page: https://www.bio-techne.com/gmp-products/cloudz-cell-activation-kits. The final product would use our Custom ordering channel.

A: While both kits have CD3/CD28antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

A:  We do not expect residual microsphers to be remaining after the Release Buffer treatment and subsequent washing step. We are in the process of developing a mass spectrometry based method to identify trace antibody and polymeric residuals in the expanded cell population. We have not visually observed any undissolved microspheres after release buffer treatment.

A: The Release Buffer will not have an effect on cell viability when used as recommended in the protocol. Cells should not, however, be left in release buffer for longer than 30 minutes, as that can impact viability.

A: The information on number of antibodies per microsphere in the Cloudz product is proprietary.  The number of antibodies per microsphere has been developed for optimal performance.

A: The Cloudz microspheres can be seen as transluscent spheres by lowering the focal plane of the microscope. For more information, contact technical support.

A: The Cloudz microspheres are made of alginate - a natural polymer derived from seaweed.

A: The composition of Cloudz Release buffer is proprietary information.

A: The information on concentration of microspheres (number of microsphers per volume) is proprietary. The concentration has been developed for optimal stimultaion.

A: The Cloudz microspheres are 2-10 µm in supplied buffer, and quickly swell to average diameter of a T cell in cell culture media.

A: The antibodies present on the microspheres supplied in CLD006 kit are monoclonal antibodies raised against human antigens and these are humanized antibodies. The monoclonal antibodies are R&D Systems' in-house clones.

A: The microspheres are made of alginate co-polymer, gelled into spherical particles in the presence of divalent salt ions.

A: The Cloudz microspheres settle down along with the cells when added to culture. This is an advantage as the cells stay in close proximity to the activating reagent.