GMP Cloudz Human Treg Cell Expansion Kit

GMP Cloudz Human Treg Cell Expansion Kit

Fold Expansion of Treg Cells using the GMP Cloudz Human Treg Cell Expansion Kit Primary human CD4⁺ cells were activated with GMP-Grade Cloudz Treg CD3/CD28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Expansion of FoxP3⁺ Treg cells was evaluated using flow cytometry on Day 5 and Day 9 and fold expansion of was calculated relative to Day 0. Error bars reflect standard deviation across 3 donors.

Fold Expansion of CD4⁺/CD25⁺/CD127^- Treg Cells using the GMP Cloudz Human Treg Cell Expansion Kit Primary human CD4⁺ cells were activated with GMP-Grade Cloudz Treg CD3/CD28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Expansion of CD4⁺/CD25⁺/CD127^- Treg cells was evaluated using flow cytometry on Day 5, 7 and 9 and fold expansion of was calculated relative to Day 0. Error bars reflect standard deviation across 3 donors.

Increased FoxP3⁺ CD4 Cells following Treg Expansion. Flow cytometry data showing expression of the Treg cell marker, FoxP3, in CD4⁺ T cells on Day 0 (A, C) and Day 9 (B, D) following expansion using the GMP Cloudz Human Treg Expansion Kit. Nine days after expansion, the cells were fixed, permeabilized, and stained with CD4-FITC, CD25-APC, and FoxP3-PE antibodies. Cells in panels A and B were stained with FoxP3 and CD4. Cells in C and D were stained with FoxP3 and CD25.

Viability greater than 90% after 9-day expansion Viability of Human Treg after expansion with GMP Cloudz Human Treg Cell Expansion Kit. Primary human CD4⁺ cells were activated with GMP-Grade Cloudz Treg CD3/28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Cell viability after expansion for 9 days was greater than 90%. Error bars reflect standard deviation across 3 donors.

Fold expansion of Treg Cells with GMP Cloudz Human Treg Cell Expansion Kit compared to other suppliers Fold expansion of CD25⁺/CD4⁺/FoxP3⁺ Treg Cells is greater with GMP Cloudz Human Treg Cell Expansion Kit compared to other GMP-Grade Treg Cell Expansion suppliers.