GOLGA4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-04456

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Cited:

Immunocytochemistry/Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GOLGA4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2). MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTEQLDEGTPNRESGDTQSFAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSSTASFDPPSDMDSEAEDLVGNSDSLNKEQLIQRLRRMERSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

261 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GOLGA4 Antibody - BSA Free

Western Blot: GOLGA4 AntibodyBSA Free [NBP3-04456]

Western Blot: GOLGA4 AntibodyBSA Free [NBP3-04456]

Western Blot: GOLGA4 Antibody [NBP3-04456] - Analysis of extracts of various cell lines, using GOLGA4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456]

Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456]

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Analysis of L929 cells using GOLGA4 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. GOLGA4 antibody
GOLGA4 Antibody

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] -

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Rat hepatocytes were stained with anti-GOLGA4 Antibody. Image from verified customer review.
GOLGA4 Antibody

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] -

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - Mice hepatocytes were stained with anti-GOLGA4 Antibody. Image from verified customer review.
GOLGA4 Antibody

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] -

Immunocytochemistry/Immunofluorescence: GOLGA4 Antibody [NBP3-04456] - HepG2 cells stained with GOLGA4 Antibody. Image from verified customer review.
GOLGA4 Antibody - BSA Free

Proximity Ligation Assay: Rabbit Polyclonal GOLGA4 Antibody [NBP3-04456]

Proximity Ligation Assay: Rabbit Polyclonal GOLGA4 Antibody [NBP3-04456] - PLA using a Rabbit anti-p230 Ab (recognizing amino acids 1-150 in the protein's N-terminus) (NBP3-04456) and Mouse Ab recognizing the central regions of p230 located within aa 1482-1584. Image from a verified customer review.
GOLGA4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] -

Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] - Immunofluorescence analysis of C6 cells using GOLGA4 Rabbit pAb (A10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GOLGA4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] -

Immunocytochemistry/ Immunofluorescence: GOLGA4 Antibody - BSA Free [NBP3-04456] - Immunofluorescence analysis of HeLa cells using GOLGA4 Rabbit pAb (A10216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for GOLGA4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Proximity Ligation Assay

Valifated for Proximity Ligation Assay from a verified customer review.

Western Blot

1:1000-1:2000

Reviewed Applications

Read 4 reviews rated 4.8 using NBP3-04456 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GOLGA4

Alternate Names

72.1 protein, GCP2, GOLG, golgin A4, golgin subfamily a, 4, golgin-240, golgin-245, MU-RMS-40.18, p230, protein 72.1

Gene Symbol

GOLGA4

Additional GOLGA4 Products

Product Documents for GOLGA4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GOLGA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GOLGA4 Antibody - BSA Free

Customer Reviews for GOLGA4 Antibody - BSA Free (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • GOLGA4 Antibody - BSA Free
    Name: Armen Petrosyan
    Application: Proximity Ligation Assay
    Sample Tested: HepG2 and HepG2 CELLS
    Species: Human
    Verified Customer | Posted 11/07/2024
    PLA using a Rabbit anti-p230 Ab (recognizing amino acids 1-150 in the protein's N-terminus) (Novus Biologicals, NBP3-04456) and Mouse Ab recognizing the central regions of p230 located within aa 1482-1584.
    The examination of the spatial relationship between p230's C- and N-termini using PLA.
    GOLGA4 Antibody - BSA Free NBP3-04456
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • GOLGA4 Antibody
    Name: Armen Petrosyan
    Application: Immunofluorescence
    Sample Tested: Rat hepatocytes
    Species: Rat
    Verified Customer | Posted 06/27/2023
    Rat hepatocytes were stained with anti-GolgA4 Ab
    GOLGA4 Antibody - BSA Free NBP3-04456
  • GOLGA4 Antibody
    Name: Armen Petrosyan
    Application: Immunofluorescence
    Sample Tested: mouse primary hepatocytes
    Species: Mouse
    Verified Customer | Posted 06/27/2023
    Mice hepatocytes were stained with anti-GolgA4 Ab.
    GOLGA4 Antibody - BSA Free NBP3-04456
  • GOLGA4 Antibody
    Name: Armen Petrosyan
    Application: Immunofluorescence
    Sample Tested: HepG2 hepatoma cells
    Species: Human
    Verified Customer | Posted 06/27/2023
    HepG2 cells stained with GolgA4 antibody
    GOLGA4 Antibody - BSA Free NBP3-04456

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