GPRC5B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87160

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GPRC5B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEHNAALRTAGFPNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPRC5B Antibody - BSA Free

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Analysis in human cerebral cortex and liver tissues. Corresponding GPRC5B RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: GPRC5B Antibody [NBP1-87160]

Immunocytochemistry/ Immunofluorescence: GPRC5B Antibody [NBP1-87160]

Immunocytochemistry/Immunofluorescence: GPRC5B Antibody [NBP1-87160] - Staining of human cell line U-251 MG shows localization to nucleus and vesicles.
Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human caudate putamen shows strong membranous positivity in glial cells.
Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human cerebral cortex shows strong membranous positivity in neuropil.
Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human kidney shows strong membranous positivity in cells in glomeruli and distal tubules.
Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human liver shows weak positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160]

Immunohistochemistry-Paraffin: GPRC5B Antibody [NBP1-87160] - Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.

Applications for GPRC5B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPRC5B

GPRC5B is an Orphan-C GPCR induced by retinoids. GPRC5B shows some homologies to the metabotropic glutamate receptor-like family of GPCRs; however, the GPRC5B protein has a short N-terminal domain compared to other family C members. GPRC5B expression is reported to be widespread with highest levels observed in brain, kidney, pancreas and testis. ESTs have been isolated from a wide variety of human tissue libraries, including normal adrenal, brain, heart/melanocyte/uterus and pineal, and cancerous germ cell, nerve, ovary and parathyroid.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member B

Alternate Names

RAIG2

Gene Symbol

GPRC5B

Additional GPRC5B Products

Product Documents for GPRC5B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GPRC5B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

Citations for GPRC5B Antibody - BSA Free

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Protocols

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