GRAIL/RNF128 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24610

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Primate

Cited:

Human, Mouse

Predicted:

Bovine (94%), Chimpanzee (100%), Monkey (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids portion of amino acids 227-269 of human GRAIL was used as immunogen, GenBank no. NP_919445.1.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in chimpanzee, monkey and orangutan, 94% homologous in cow, 88% homologous in cat and 83% homologous in opossum, rat and mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRAIL/RNF128 Antibody - BSA Free

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

GRAIL-RNF128-Antibody-Western-Blot-NBP2-24610-img0006.jpg
Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610]

Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610]

Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using NBP2-24610 at 1 ug/ml.
GRAIL/RNF128 Antibody

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] -

Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Bone marrow progenitors were isolated from C57BL/6 mice & incubated with 20 ng/ml GM-CSF. (a) Cells were collected at days 0, 2, 4, 6, 7, 8, or 9. A portion of the cells at day 8 were stimulated with OVA for 24 h. Collected cells were processed for protein extraction followed by immunoblot analysis with antibodies to the indicated proteins. Blots were stripped of antibodies prior to probing with the next one. The two braces on the left represent two different gels of the same samples. (b) Bone marrow progenitors from WT or PARP-1−/− mice were isolated & processed as in (a). Protein extracts were subjected to immunoblot analysis with antibodies to the indicated proteins. For (a, b), signals were quantified & are shown as values under the respective blots. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31178661), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GRAIL/RNF128 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24356810)

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRAIL/RNF128

The protein encoded by the RNF128 gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128

Alternate Names

RNF128

Gene Symbol

RNF128

UniProt

Additional GRAIL/RNF128 Products

Product Documents for GRAIL/RNF128 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GRAIL/RNF128 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRAIL/RNF128 Antibody - BSA Free

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Protocols

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