GRAIL/RNF128 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-24610
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Equine, Primate
Cited:
Human, Mouse
Predicted:
Bovine (94%), Chimpanzee (100%), Monkey (100%), Orangutan (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids portion of amino acids 227-269 of human GRAIL was used as immunogen, GenBank no. NP_919445.1.
Reactivity Notes
The amino acid sequence used as immunogen is 100% homologous in chimpanzee, monkey and orangutan, 94% homologous in cow, 88% homologous in cat and 83% homologous in opossum, rat and mouse.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GRAIL/RNF128 Antibody - BSA Free
Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610]
Immunohistochemistry-Paraffin: GRAIL/RNF128 Antibody [NBP2-24610] - Staining of normal human brain using control (rabbit Ig) at 5 ug/ml.Western Blot: GRAIL/RNF128 Antibody [NBP2-24610]
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Analysis of human GRAIL in human spleen lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse spleen and 4) rat spleen using NBP2-24610 at 1 ug/ml.Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] -
Western Blot: GRAIL/RNF128 Antibody [NBP2-24610] - Bone marrow progenitors were isolated from C57BL/6 mice & incubated with 20 ng/ml GM-CSF. (a) Cells were collected at days 0, 2, 4, 6, 7, 8, or 9. A portion of the cells at day 8 were stimulated with OVA for 24 h. Collected cells were processed for protein extraction followed by immunoblot analysis with antibodies to the indicated proteins. Blots were stripped of antibodies prior to probing with the next one. The two braces on the left represent two different gels of the same samples. (b) Bone marrow progenitors from WT or PARP-1−/− mice were isolated & processed as in (a). Protein extracts were subjected to immunoblot analysis with antibodies to the indicated proteins. For (a, b), signals were quantified & are shown as values under the respective blots. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31178661), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GRAIL/RNF128 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 24356810)
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.5-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRAIL/RNF128
Long Name
Gene Related to Anergy in Lymphocytes Protein/Ring Finger Protein 128
Alternate Names
RNF128
Gene Symbol
RNF128
UniProt
Additional GRAIL/RNF128 Products
Product Documents for GRAIL/RNF128 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GRAIL/RNF128 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for GRAIL/RNF128 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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