Granzyme B Antibody (BLR022E)

Name: Granzyme B Antibody (BLR022E)
Brand: Novus Biologicals
SKU: NBP2-76414
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-76414

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR022E

View all Granzyme B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein representing Human Granzyme B

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granzyme B Antibody (BLR022E)

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414]

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414] - Myc-tagged Granzyme H and B overexpressing cell lysate (15 ug) prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-myc antibody.

Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of Granzyme B over-expressing cells (left) and Granzyme H overexpressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of lung carcinoma. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG.

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414]

Western Blot: Granzyme B Antibody (BLR022E) [NBP2-76414] - Whole cell lysate (15 ug) from HEK293T, HeLa, SR, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GranzymeB recombinantmonoclonal antibody [BLR022E] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunohistochemistry: Granzyme B Antibody (BLR022E) [NBP2-76414] - Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] used at 1:250. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414]

Immunohistochemistry-Paraffin: Granzyme B Antibody (BLR022E) [NBP2-76414] - FFPE section of lymph node. Antibody: Rabbit anti-Granzyme B recombinantmonoclonal antibody [BLR022E]. Secondary: HRP-conjugated goat anti-rabbit IgG

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Applications for Granzyme B Antibody (BLR022E)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Multiplex Immunofluorescence

1:250

Western Blot

1:1000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Granzyme B

Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Gene Symbol

GZMB

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Product Documents for Granzyme B Antibody (BLR022E)

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Certificate of Analysis

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Product Specific Notices for Granzyme B Antibody (BLR022E)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.