Granzyme B Antibody - BSA Free

Name: Granzyme B Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-88151
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-88151

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granzyme B Antibody - BSA Free

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining in human lymph node and skeletal muscle tissues using NBP1-88151 antibody. Corresponding GZMB RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]

Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

Western Blot: Granzyme B Antibody - BSA Free [NBP1-88151]

Analysis in control (vector only transfected HEK293T lysate) and gZMB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

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Applications for Granzyme B Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. ICC/IF reactivity reported in scientific literature (PMID: 22198309).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Gene Symbol

GZMB

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Product Specific Notices for Granzyme B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.