GRK2 Antibody (3F8)

Novus Biologicals | Catalog # NBP2-37611

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F8
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Product Specifications

Immunogen

Purified recombinant fragment of human GRK2 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse GRK2 Antibody (3F8) (NBP2-37611) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-GRK2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GRK2 Antibody (3F8)

Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

GRK2-Antibody-3F8-Immunohistochemistry-NBP2-37611-img0006.jpg
Western Blot: GRK2 Antibody (3F8)BSA Free [NBP2-37611]

Western Blot: GRK2 Antibody (3F8)BSA Free [NBP2-37611]

Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

GRK2-Antibody-3F8-Immunohistochemistry-NBP2-37611-img0007.jpg
Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.
ELISA: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

ELISA: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]

ELISA: GRK2 Antibody (3F8) [NBP2-37611] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
GRK2 Antibody (3F8) - BSA Free

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] -

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2), beta -arrestin2, & 8-hydroxy-2′deoxyguanosine (8-OHdG) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute & recovery phases. Micrographs showing immunofluorescence stainings of GRK2 & beta -arrestin2, & immunohistochemical staining of 8-OHdG in the acute & recovery phases. Arrowheads (yellow) indicate positive signals for GRK2 & beta -arrestin2 on the cell membrane, & Arrowheads (red) indicate 8-OHdG-positive nuclei. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GRK2 Antibody (3F8) - BSA Free

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] -

Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) & dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4′,6-diamidino-2-phenylindole (DAPI). (b) Quantification for GRK2 positive stained area in the myocardium. The box represents the 25th & 75th percentiles & the line the median value. Whiskers correspond to the 25th percentile minus 1.5 times interquartile range (IQR) & to the 75th percentile plus 1.5 IQR. (c) Quantification for membranous GRK2 positive cardiomyocyte. *P < 0.001 vs. the normal control (NC) group. †P < 0.001 vs. the DCM group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for GRK2 Antibody (3F8)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRK2

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Long Name

G protein-coupled Receptor Kinase 2

Alternate Names

ADRBK1, BARK1, Beta-ARK1

Gene Symbol

GRK2

Additional GRK2 Products

Product Documents for GRK2 Antibody (3F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GRK2 Antibody (3F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for GRK2 Antibody (3F8)

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Protocols

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