GRK2 Antibody (3F8)
Novus Biologicals | Catalog # NBP2-37611
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey, Primate
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3F8
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Product Specifications
Immunogen
Purified recombinant fragment of human GRK2 expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse GRK2 Antibody (3F8) (NBP2-37611) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-GRK2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GRK2 Antibody (3F8)
Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
GRK2-Antibody-3F8-Immunohistochemistry-NBP2-37611-img0006.jpgWestern Blot: GRK2 Antibody (3F8)BSA Free [NBP2-37611]
Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
GRK2-Antibody-3F8-Immunohistochemistry-NBP2-37611-img0007.jpgImmunohistochemistry-Paraffin: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.ELISA: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
ELISA: GRK2 Antibody (3F8) [NBP2-37611] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] -
Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2), beta -arrestin2, & 8-hydroxy-2′deoxyguanosine (8-OHdG) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute & recovery phases. Micrographs showing immunofluorescence stainings of GRK2 & beta -arrestin2, & immunohistochemical staining of 8-OHdG in the acute & recovery phases. Arrowheads (yellow) indicate positive signals for GRK2 & beta -arrestin2 on the cell membrane, & Arrowheads (red) indicate 8-OHdG-positive nuclei. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] -
Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) & dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4′,6-diamidino-2-phenylindole (DAPI). (b) Quantification for GRK2 positive stained area in the myocardium. The box represents the 25th & 75th percentiles & the line the median value. Whiskers correspond to the 25th percentile minus 1.5 times interquartile range (IQR) & to the 75th percentile plus 1.5 IQR. (c) Quantification for membranous GRK2 positive cardiomyocyte. *P < 0.001 vs. the normal control (NC) group. †P < 0.001 vs. the DCM group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for GRK2 Antibody (3F8)
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRK2
Long Name
G protein-coupled Receptor Kinase 2
Alternate Names
ADRBK1, BARK1, Beta-ARK1
Gene Symbol
GRK2
Additional GRK2 Products
Product Documents for GRK2 Antibody (3F8)
Product Specific Notices for GRK2 Antibody (3F8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for GRK2 Antibody (3F8)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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