GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05)

Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes

Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes

Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate

Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.