gtf3c3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35833

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all gtf3c3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human gtf3c3 (NP_036218.1).

Sequence:
MSGFSPELIDYLEGKISFEEFERRREERKTREKKSLQEKGKLSAEENPDDSEVPSSSGINSTKSQDKDVNEGETSDGVRKSVHKVFASMLGENEDDEEEEEEEEEEEEEEETPEQPTAGDVFVLEMVLNRETKKMMKEKRPRSKLPRALRGLMGEANIRF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for gtf3c3 Antibody - BSA Free

gtf3c3 Antibody

Immunocytochemistry/ Immunofluorescence: gtf3c3 Antibody [NBP3-35833] -

Immunocytochemistry/ Immunofluorescence: gtf3c3 Antibody [NBP3-35833] - Immunofluorescence analysis of U2OS cells using gtf3c3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
gtf3c3 Antibody

Immunocytochemistry/ Immunofluorescence: gtf3c3 Antibody [NBP3-35833] -

Immunocytochemistry/ Immunofluorescence: gtf3c3 Antibody [NBP3-35833] - Immunofluorescence analysis of H9C2 cells using gtf3c3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
gtf3c3 Antibody

Immunohistochemistry: gtf3c3 Antibody [NBP3-35833] -

Immunohistochemistry: gtf3c3 Antibody [NBP3-35833] - Immunohistochemistry analysis of paraffin-embedded Rat brain using gtf3c3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
gtf3c3 Antibody

Western Blot: gtf3c3 Antibody [NBP3-35833] -

Western Blot: gtf3c3 Antibody [NBP3-35833] - Western blot analysis of various lysates using gtf3c3 Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
gtf3c3 Antibody

Immunohistochemistry: gtf3c3 Antibody [NBP3-35833] -

Immunohistochemistry: gtf3c3 Antibody [NBP3-35833] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using gtf3c3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for gtf3c3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: gtf3c3

General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.

Alternate Names

general transcription factor 3C polypeptide 3, general transcription factor IIIC, polypeptide 3, 102kDa, TF3C-gamma, TFIIIC 102 kDa subunit, TFIIIC102general transcription factor IIIC, polypeptide 3 (102kD), TFiiiC2-102, TFIIICgamma, Transcription factor IIIC 102 kDa subunit, Transcription factor IIIC subunit gamma, transcription factor IIIC, 102 kD

Gene Symbol

GTF3C3

Additional gtf3c3 Products

Product Documents for gtf3c3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for gtf3c3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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