H4 Clustered Histone 1 [ac Lys12] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35422

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all H4 Clustered Histone 1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic acetylated peptide around K12 of human H4 Clustered Histone 1 (NP_001029249.1).

Sequence:
GLGKG

Modification

ac Lys 12

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for H4 Clustered Histone 1 [ac Lys12] Antibody - BSA Free

H4 Clustered Histone 1 [ac Lys12] Antibody

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunofluorescence analysis of C6 cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.
H4 Clustered Histone 1 [ac Lys12] Antibody

Western Blot: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Western Blot: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:500 dilution. HeLa cells and C6 cells were treated by TSA (1 uM) at 37C for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
H4 Clustered Histone 1 [ac Lys12] Antibody

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunofluorescence analysis of NIH/3T3 cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.
H4 Clustered Histone 1 [ac Lys12] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
H4 Clustered Histone 1 [ac Lys12] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunohistochemistry analysis of paraffin-embedded Human appendix using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
H4 Clustered Histone 1 [ac Lys12] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
H4 Clustered Histone 1 [ac Lys12] Antibody

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -

Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for H4 Clustered Histone 1 [ac Lys12] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: H4 Clustered Histone 1

Alternate Names

H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA, H4F4, HIST1H4A

Gene Symbol

H4C1

Additional H4 Clustered Histone 1 Products

Product Documents for H4 Clustered Histone 1 [ac Lys12] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for H4 Clustered Histone 1 [ac Lys12] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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