HARS2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81775

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HARS2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AVRCQSQVAEAVLTSQLKAHQEKPN

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HARS2 Antibody - BSA Free

Western Blot: HARS2 Antibody [NBP1-81775]

Western Blot: HARS2 Antibody [NBP1-81775]

Western Blot: HARS2 Antibody [NBP1-81775] - Analysis in control (vector only transfected HEK293T lysate) and HARS2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: HARS2 Antibody [NBP1-81775]

Immunocytochemistry/ Immunofluorescence: HARS2 Antibody [NBP1-81775]

Immunocytochemistry/Immunofluorescence: HARS2 Antibody [NBP1-81775] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry: HARS2 Antibody [NBP1-81775]

Immunohistochemistry: HARS2 Antibody [NBP1-81775]

Immunohistochemistry: HARS2 Antibody [NBP1-81775] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Applications for HARS2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HARS2

The HARS2 gene encodes a 506 amino acid, 56kDA probable histidine--tRNA ligase (mitochondrial) protein that is member to the II family of aminoacyl-tRNA synthetases which charge tRNA with cognate amino acids. The HARS2 gene is located in a head-to-head orientation with HARS on chromosome 5, which is the location of where homologous genes share a bidirectional promoter. The protein works as an accessory in the regulation of protein biosynthesis through the synthesis of histidyl-transfer RNA. HARS2 is involved in gene expression and mitochondrian tRNA aminoacylation and has been known to interact with genes ICT1, AARS2, AASS, ABSB7, and ACAD9. HARS2 has been investigated in immunodeficiency, malaria, pneumonia, hearing loss, tuberculosis, schizophrenia, and ovarian dysgenesis.

Alternate Names

EC 6.1.1, EC 6.1.1.21, HARSLprobable histidyl-tRNA synthetase, mitochondrial, HARS-related, HARSRhistidine tRNA ligase 2, mitochondrial (putative), hisRS, histidine translase, Histidine--tRNA ligase, Histidine--tRNA ligase-like, histidyl-tRNA synthetase 2, mitochondrial (putative), histidyl-tRNA synthetase-like, HO3histidine-tRNA ligase homolog

Entrez Gene IDs

23438 (Human)

Gene Symbol

HARS2

UniProt

P49590 (Human)

Additional HARS2 Products

Product Documents for HARS2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HARS2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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