HCA59 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-83168
![Western Blot: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Western-Blot-NBP1-83168-img0015.jpg "Western Blot: HCA59 Antibody [NBP1-83168]")
Loading...
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKE
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23276153)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HCA59 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83168-img0008.jpg "Immunocytochemistry/ Immunofluorescence: HCA59 Antibody [NBP1-83168]")
Immunocytochemistry/ Immunofluorescence: HCA59 Antibody [NBP1-83168]
Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-83168] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0025.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.![Western Blot: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Western-Blot-NBP1-83168-img0007.jpg "Western Blot: HCA59 Antibody [NBP1-83168]")
Western Blot: HCA59 Antibody [NBP1-83168]
Western Blot: HCA59 Antibody [NBP1-83168] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![Western Blot: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Western-Blot-NBP1-83168-img0012.jpg "Western Blot: HCA59 Antibody [NBP1-83168]")
![Western Blot: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Western-Blot-NBP1-83168-img0013.jpg "Western Blot: HCA59 Antibody [NBP1-83168]")
Western Blot: HCA59 Antibody [NBP1-83168]
Western Blot: HCA59 Antibody [NBP1-83168] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0002.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human gall bladder shows strong nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0009.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human bone marrow shows high expression.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0010.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human pancreas shows low expression as expected.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0016.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human testis.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0017.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human kidney.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0018.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human lymph node.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0019.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0021.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human bone marrow, fallopian tube, lymph node and testis using Anti-HCA59 antibody NBP1-83168 (A) shows similar protein distribution across tissues to independent antibody NBP1-83170 (B).![Immunohistochemistry: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-NBP1-83168-img0022.jpg "Immunohistochemistry: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry: HCA59 Antibody [NBP1-83168]
Immunohistochemistry: HCA59 Antibody [NBP1-83168] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0023.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.![Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]](https://resources.rndsystems.com/images/products/HCA59-Antibody-Immunohistochemistry-Paraffin-NBP1-83168-img0024.jpg "Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]")
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.Applications for HCA59 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HCA59
Alternate Names
bA409K20.3, C9orf78, chromosome 9 open reading frame 78, HCA59, Hepatocellular carcinoma-associated antigen 59, HSPC220
Gene Symbol
C9ORF78
Additional HCA59 Products
Product Documents for HCA59 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HCA59 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HCA59 Antibody - BSA Free
Powered by Bioz
Customer Reviews for HCA59 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review HCA59 Antibody - BSA Free and earn rewards!
Have you used HCA59 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...