HDAC3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31196

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 3/HDAC3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC3 Antibody - BSA Free

Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196]

Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196]

Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Chromatin Immunoprecipitation: HDAC3 Antibody [NBP1-31196] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196] - Analysis of on: A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000
Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 10% SDS-PAGE gel.
Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196]

Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
HDAC3 Antibody

Western Blot: HDAC3 Antibody [NBP1-31196] -

Western Blot: HDAC3 Antibody [NBP1-31196] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.
HDAC3 Antibody

Western Blot: HDAC3 Antibody [NBP1-31196] -

Western Blot: HDAC3 Antibody [NBP1-31196] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.
HDAC3 Antibody

Western Blot: HDAC3 Antibody [NBP1-31196] -

Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
HDAC3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NBP1-31196] -

HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:400.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.
HDAC3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NBP1-31196] -

HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for HDAC3 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Gene Symbol

HDAC3

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HDAC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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