Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Western Blot, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Histone Deacetylase 5/HDAC5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]

Reactivity Notes

Rat reactivity reported in scientific literature (Kim et al).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit HDAC5 Antibody - BSA Free (NBP2-22152) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and ChIP. Anti-HDAC5 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HDAC5 Antibody - BSA Free

Western Blot: HDAC5 AntibodyBSA Free [NBP2-22152]

Western Blot: HDAC5 AntibodyBSA Free [NBP2-22152]

Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunohistochemistry: HDAC5 Antibody - BSA Free [NBP2-22152]

Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.
Western Blot: HDAC5 AntibodyBSA Free [NBP2-22152]

Western Blot: HDAC5 AntibodyBSA Free [NBP2-22152]

Western Blot: HDAC5 Antibody [NBP2-22152] - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.

Applications for HDAC5 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 32196931)

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1.0 - 2.0 ug/ml
Application Notes
In Western blot a band was observed ~ 130 kDa. In ICC/IF nuclear staining was observed in A431 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP2-22152 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 5/HDAC5

HDAC5, or histone deacetylase mHDA1, is responsible for the deacetylation of lysine residues on the N-terminal end of the core histones H2A, H2B, H3 and H4. Identifying histone acetyltransferases reveals much needed insight on how these enzymes regulate transcription, and further elucidates their pivotal role in cell cycle progression and other developmental events. HDAC5 shuttles between the cytoplasm and nucleus, depending on its nuclear export sequence, and shares interactions with BAHD1, KDM5B and MEF family proteins. While HDAC5 has many biological implications, recent studies have proposed its key involvement in synaptic plasticity and memory formation (PMID: 22914591), as well as its connection to various cancers (PMID: 22301920, PMID: 22944197).

Long Name

Histone Deacetylase 5

Alternate Names

FLJ90614, KIAA0600, NY-CO-9

Entrez Gene IDs

10014 (Human)

Gene Symbol

HDAC5

UniProt

Q9UQL6 (Human)

Additional Histone Deacetylase 5/HDAC5 Products

Product Documents for HDAC5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HDAC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Epigenetics

Citations for HDAC5 Antibody - BSA Free

Customer Reviews for HDAC5 Antibody - BSA Free (1)

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  • Name: Liyong Zhang
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/15/2014
    A review for HDAC5 antibody (NBP2-22152)
    HDAC5 Antibody - BSA Free NBP2-22152

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Protocols

View specific protocols for HDAC5 Antibody - BSA Free (NBP2-22152):

HDAC5 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

HDAC5 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.


HDAC5 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 25 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute anti-HDAC5 primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HDAC5 Antibody - BSA Free

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    • Q: Do the following antibodies  cross-react with any other the HDAC's? 1) NBP2-22152 and 2) NBP2-16795
      A: We have not actually tested these for cross reactivity.   I have looked at homology between he HDAC5/HDAC7 proteins and they are generally only about 60% homologous. As polyclonal antibodies they may cross react slightly.
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