Hemopexin Antibody (1M6O3)

Novus Biologicals | Catalog # NBP3-16762

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1M6O3 expressed in HEK293
View all Hemopexin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 363-462 of human Hemopexin (P02790). AFICPGSSRLHIMAGRRLWWLDLKSGAQATWTELPWPHEKVDGALCMEKSLGPNSCSANGPGLYLIHGPNLYCYSDVEKLNAAKALPQPQNVTSLLGCTH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hemopexin Antibody (1M6O3)

Western Blot: Hemopexin Antibody (1M6O3) [NBP3-16762]

Western Blot: Hemopexin Antibody (1M6O3) [NBP3-16762]

Western Blot: Hemopexin Antibody (1M6O3) [NBP3-16762] - Western blot analysis of extracts of Human serum, using Hemopexin (HPX) Rabbit mAb (NBP3-16762) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Hemopexin Antibody (1M6O3) [NBP3-16762]

Immunohistochemistry-Paraffin: Hemopexin Antibody (1M6O3) [NBP3-16762]

Immunohistochemistry-Paraffin: Hemopexin Antibody (1M6O3) [NBP3-16762] - Immunohistochemistry of paraffin-embedded rat liver using Hemopexin (HPX) Rabbit mAb (NBP3-16762) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Hemopexin Antibody (1M6O3)

Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [NBP3-16762] -

Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [NBP3-16762] - Confocal imaging of paraffin-embedded Rat liver using Hemopexin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Hemopexin Antibody (1M6O3)

Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [NBP3-16762] -

Immunocytochemistry/ Immunofluorescence: Hemopexin Antibody (1M6O3) [NBP3-16762] - Confocal imaging of paraffin-embedded Human liver using Hemopexin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for Hemopexin Antibody (1M6O3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hemopexin

The Hemopexin gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phaseprotein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidativestress. (provided by RefSeq)

Alternate Names

Beta-1B-glycoprotein, HPX

Gene Symbol

HPX

Additional Hemopexin Products

Product Documents for Hemopexin Antibody (1M6O3)

Certificate of Analysis

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Product Specific Notices for Hemopexin Antibody (1M6O3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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