Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

Novus Biologicals | Catalog # NB110-68095

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SE-1

Format

BSA Free
View all Hepatic Sinusoidal Endothelial Cells Primary Antibodies »

Product Specifications

Immunogen

Rat Hepatic Sinusoidal Endothelial Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Western-Blot-NB110-68095-img0009.jpg
Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Immunocytochemistry-Immunofluorescence-NB110-68095-img0010.jpg
Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Flow-Cytometry-NB110-68095-img0011.jpg
Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review.
Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095]

Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.
Hepatic Sinusoidal Endothelial Cells Antibody (SE-1)

Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] -

Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [NB110-68095] - Expression of scavenger receptors & immune lectins in rat LSECs & KCs. a. Unscaled heatmaps of normalized log2 expression values (log2 (RPKM+ 1), & log2 (iBAQ+ 1)) for scavenger receptors (SR) & C-type lectins in the KC & LSEC transcriptomes & proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC & LSEC transcriptomes & proteomes. The bar height reflects good correlation between the transcriptome & proteome data for gene products of Clec4g, Clec4m, Stab1, & Stab2 in both cell types. The abundance of gene products of Marco & Cd5l were well correlated between the KC transcriptome & proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs & C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, & double immune-labeled with antibodies to Fc gamma RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), & to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green & red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 & Fc gamma RIIb2 (SE-1) specifically labeled LSECs & the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, & SR-B1 was observed in both LSECs & KCs Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33246411), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free [NB110-68095] -

Expression of scavenger receptors and immune lectins in rat LSECs and KCs. a. Unscaled heatmaps of normalized log2 expression values (log2 (RPKM+ 1), and log2 (iBAQ+ 1)) for scavenger receptors (SR) and C-type lectins in the KC and LSEC transcriptomes and proteomes. Underlined: Genes expressed in the transcriptome that were also present in the proteome. b. Absolute abundance of selected SR gene products in the KC and LSEC transcriptomes and proteomes. The bar height reflects good correlation between the transcriptome and proteome data for gene products of Clec4g, Clec4m, Stab1, and Stab2 in both cell types. The abundance of gene products of Marco and Cd5l were well correlated between the KC transcriptome and proteome, while LSECs showed high abundance of these gene products only at mRNA level. c. Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs and C-type lectins. NPCs from the 25–45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, and double immune-labeled with antibodies to Fc gamma RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), and to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green and red fluorescence is seen as yellow staining in the overlay images. Antibodies are listed in Table 1. Cell nuclei were stained with DAPI (blue). Arrow heads point to CD68 positive KCs. Antibodies to stabilin-2 and Fc gamma RIIb2 (SE-1) specifically labeled LSECs and the CD68-antibody specifically labeled KCs, whereas positive labeling for the mannose receptor, SR-A1, and SR-B1 was observed in both LSECs and KCs Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33246411), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 9428229)

Immunohistochemistry

1-5 ug/ml

Immunohistochemistry-Frozen

1-5 ug/ml

Immunohistochemistry-Paraffin

1-5 ug/ml

Western Blot

1-5 ug/ml
Application Notes
In WB, Hepatic Sinusoidal Endothelial Cells antibody (clone SE-1) generates a specific band around 45-50 kDa molecular weight position. For IHC use neutral buffered formalin fixated (perfusion fixation is recommended) paraffin embedded tissues after proteinase K treatment or acetone fixed frozen sections.

Reviewed Applications

Read 3 reviews rated 3.7 using NB110-68095 in the following applications:

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hepatic Sinusoidal Endothelial Cells

Hepatic sinusoidal endothelial cells (HSEC) plays a key role in various hepatic pathophysiological conditions and are known for their morphological uniqueness - numerous open fenestrations clustered in groups (sieve plates), lack of distinct basal lamina, and abundant vacuolar/vesicular structures. HSECs are distinct from vascular endothelial cells functionally/morphologically and besides providing sinusoidal barrier, they implicates in the balance or distribution of lipids, cholesterol, and vitamin A, and in the uptake of various substances via their specialized endocytotic mechanisms. SE-1 monoclonal antibody has been specifically designed to recognize an antigen expressed in HSEC, but not in other cell types including vascular endothelial cells. Experiments on developing liver have revealed that the expression of SE-1 antigen is possibly regulated via intimate association of HSECs with hepatocytes. Hepatic sinusoidal endothelial cells (SE-1) antibody has been extensively used as one of the most reliable markers for rat HSEC by Western blot and immunohistochemistry analysis and is a useful tool for studying the role of HSE in various hepato-pathophysiological conditions.

Alternate Names

Hepatic cells, Hepatic Sinusoidal Endothelial Cells, HSE, SE1, Sinusoidal Endothelial Cells

Additional Hepatic Sinusoidal Endothelial Cells Products

Product Documents for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free

Customer Reviews for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Hepatic Sinusoidal Endothelial Cells Antibody (SE-1)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen Rat Liver Tissue sections
    Species: Rat
    Verified Customer | Posted 10/10/2019
    DAPI (blue), SE1 (green), cytochrome P450 (red)
    Normal rat liver Acetone fixation SE1 antibody used at 1:500 dilution, incubated at 4C overnight Multiplexed with cytochrome P450 antibody from another vendor
    Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free NB110-68095
  • Name: Fabio Marongiu
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen Rat Liver Tissue sections
    Species: Rat
    Verified Customer | Posted 10/15/2015
    Normal Rat Liver - SE-1 (green) and DAPI (blue)
    Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - BSA Free NB110-68095
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 20400698
    Species: Mouse
    Verified Customer | Posted 12/29/2014

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