Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80759

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SE-1

Format

Azide and BSA Free
View all Hepatic Sinusoidal Endothelial Cells Primary Antibodies »

Product Specifications

Immunogen

Rat Hepatic Sinusoidal Endothelial Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1)Azide and BSA Free [NBP2-80759]

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1)Azide and BSA Free [NBP2-80759]

Western Blot: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Total protein from human, mouse and rat liver was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-H.S.E.C in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Immunocytochemistry/ Immunofluorescence: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Hepatic-Sinusoidal-Endothelial-Cells-Antibody-SE-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80759-img0005.jpg
Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Immunohistochemistry-Frozen: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Frozen rat liver tissue sections. DAPI (blue), SE1 (green), cytochrome P450 (red). Tissue sections were acetone fixed. SE1 antibody at 1:500, incubated at 4C overnight. Multiplexed with cytochrome P450 from another vendor. IHC-Fr image submitted by a veri
Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Flow Cytometry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Using the FITC direct conjugate Flow Cytometry: Surface staining of Rat Liver sinusoidal endothelial cells with Mouse anti-Rat Hepatic Sinusoidal Endothelial Cells [FITC] [NB110-68095F] and negative control. Total viable cells were used for analysis. Image courtesy of customer.
Immunohistochemistry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Immunohistochemistry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759]

Immunohistochemistry: Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) - Azide and BSA Free [NBP2-80759] - Analysis of frozen normal rat liver tissue sections using anti-Hepatic Sinusoidal Endothelial Cells antibody (green). Nuclei were counterstained with DAPI (blue). Image from verified customer review. Image from the standard format of this antibody.

Applications

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (Motoyama et al; PMID: 9428229)

Immunohistochemistry

1-5 ug/ml

Immunohistochemistry-Frozen

1-5 ug/ml

Immunohistochemistry-Paraffin

1-5 ug/ml

Western Blot

1-5 ug/ml
Application Notes
In WB, Hepatic Sinusoidal Endothelial Cells antibody (clone SE-1) generates a specific band around 45-50 kDa molecular weight position. For IHC use neutral buffered formalin fixated (perfusion fixation is recommended) paraffin embedded tissues after proteinase K treatment or acetone fixed frozen sections.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hepatic Sinusoidal Endothelial Cells

Hepatic sinusoidal endothelial cells (HSEC) plays a key role in various hepatic pathophysiological conditions and are known for their morphological uniqueness - numerous open fenestrations clustered in groups (sieve plates), lack of distinct basal lamina, and abundant vacuolar/vesicular structures. HSECs are distinct from vascular endothelial cells functionally/morphologically and besides providing sinusoidal barrier, they implicates in the balance or distribution of lipids, cholesterol, and vitamin A, and in the uptake of various substances via their specialized endocytotic mechanisms. SE-1 monoclonal antibody has been specifically designed to recognize an antigen expressed in HSEC, but not in other cell types including vascular endothelial cells. Experiments on developing liver have revealed that the expression of SE-1 antigen is possibly regulated via intimate association of HSECs with hepatocytes. Hepatic sinusoidal endothelial cells (SE-1) antibody has been extensively used as one of the most reliable markers for rat HSEC by Western blot and immunohistochemistry analysis and is a useful tool for studying the role of HSE in various hepato-pathophysiological conditions.

Alternate Names

Hepatic cells, Hepatic Sinusoidal Endothelial Cells, HSE, SE1, Sinusoidal Endothelial Cells

Additional Hepatic Sinusoidal Endothelial Cells Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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