HES-1 Antibody (SC06-21)
Novus Biologicals | Catalog # NBP2-67642
Recombinant Monoclonal Antibody
![Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Western-Blot-NBP2-67642-img0005.jpg "Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SC06-21 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human HES-1 aa 298-241 / 280. (SwissProt: Q14469 Human; SwissProt: P35428 Mouse; SwissProt: Q04666 Rat)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HES-1 Antibody (SC06-21)
Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642]
Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: SK-Br-3 cell lysate![Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Immunocytochemistry-Immunofluorescence-NBP2-67642-img0003.jpg "Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]")
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0007.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]")
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HES-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Flow Cytometry: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Flow-Cytometry-NBP2-67642-img0001.jpg "Flow Cytometry: HES-1 Antibody (SC06-21) [NBP2-67642]")
Flow Cytometry: HES-1 Antibody (SC06-21) [NBP2-67642]
Flow Cytometry: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Immunocytochemistry-Immunofluorescence-NBP2-67642-img0002.jpg "Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]")
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0004.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]")
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]](https://resources.rndsystems.com/images/products/HES-1-Antibody-SC06-21-Immunohistochemistry-Paraffin-NBP2-67642-img0006.jpg "Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]")
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded rat brain tissue using anti-Hes1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for HES-1 Antibody (SC06-21)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:500-1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HES-1
Long Name
Hairy And Enhancer of Split 1
Alternate Names
HES1, HL, HRY
Gene Symbol
HES1
Additional HES-1 Products
Product Documents for HES-1 Antibody (SC06-21)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HES-1 Antibody (SC06-21)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HES-1 Antibody (SC06-21)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Notch Signaling Pathways
