Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5E8

Format

BSA Free
View all Hexanoyl-Lysine adduct Primary Antibodies »

Product Specifications

Immunogen

Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Hexanoyl-Lysine adduct Antibody (5E8) - BSA Free

Western Blot: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Western Blot: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Western Blot: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (NBP2-59363). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (NBP2-59363) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Immunocytochemistry/ Immunofluorescence: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Immunocytochemistry/ Immunofluorescence: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Immunocytochemistry/Immunofluorescence: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (NBP2-59363). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (NBP2-59363) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Flow Cytometry: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Flow Cytometry: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Flow Cytometry: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363] - Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (NBP2-59363). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (NBP2-59363) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG.
Western Blot: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Western Blot: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Western Blot: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363] - Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8 (NBP2-59363). Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (NBP2-59363) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Immunocytochemistry/ Immunofluorescence: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363]

Immunocytochemistry/Immunofluorescence: Hexanoyl-Lysine adduct Antibody (5E8) [NBP2-59363] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.

Applications for Hexanoyl-Lysine adduct Antibody (5E8) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Hexanoyl-Lysine adduct

Alternate Names

HEL;HEL Adduct;Hexanoyl-Lys;Hexanoyl-Lys (HEL);Hexanoyl-Lys adduct

Additional Hexanoyl-Lysine adduct Products

Product Documents for Hexanoyl-Lysine adduct Antibody (5E8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Hexanoyl-Lysine adduct Antibody (5E8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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