hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-174

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Monkey

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 5E10

Format

Azide and BSA Free
View all hHpr1-p84-Thoc1 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 15-374 of human p84 expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear Marker

Specificity

This is specific for p84.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174]

Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr1-p84-Thoc1 antibody [5E10] (NB100-174) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

0.5 - 2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:100-1:500

Western Blot

0.3 - 2 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size

Reviewed Applications

Read 1 review rated 5 using NB100-174 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hHpr1-p84-Thoc1

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Alternate Names

HPR1THO complex subunit 1, hTREX84, Nuclear matrix protein p84, P84, P84N5, THO complex 1, Tho1

Entrez Gene IDs

9984 (Human)

Gene Symbol

THOC1

Additional hHpr1-p84-Thoc1 Products

Product Documents for hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free

Customer Reviews for hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T cell lysate
    Species: Human
    Verified Customer | Posted 07/25/2014
    p84 western in 293T cells
    hHpr1-p84-Thoc1 Antibody (5E10) - Azide and BSA Free NB100-174

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Protocols

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