HIGD1A Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03930

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all HIGD1A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2). MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIGD1A Antibody - Azide and BSA Free

Western Blot: HIGD1A AntibodyAzide and BSA Free [NBP3-03930]

Western Blot: HIGD1A AntibodyAzide and BSA Free [NBP3-03930]

Western Blot: HIGD1A Antibody [NBP3-03930] - Analysis of extracts of various cell lines, using HIGD1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunocytochemistry/ Immunofluorescence: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]

Immunocytochemistry/ Immunofluorescence: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]

Immunocytochemistry/Immunofluorescence: HIGD1A Antibody [NBP3-03930] - Analysis of U-2 OS cells using HIGD1A antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]

Immunohistochemistry-Paraffin: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]

Immunohistochemistry-Paraffin: HIGD1A Antibody [NBP3-03930] - Human colon carcinoma using HIGD1A antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for HIGD1A Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HIGD1A

Alternate Names

7420700H20Rik, DKFZP564K247, HIG1 domain family member 1A, HIG1 domain family, member 1A, HIG1 hypoxia inducible domain family, member 1A, HIG1DKFZp564K247, hypoxia inducible gene 1,2210020B17Rik, Hypoxia-inducible gene 1 protein

Gene Symbol

HIGD1A

Additional HIGD1A Products

Product Documents for HIGD1A Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HIGD1A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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