HIST1H3A Antibody

Novus Biologicals | Catalog # NBP2-16841

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HIST1H3A Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys9 of human HIST1H3A. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIST1H3A Antibody

Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841]

Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841]

Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded rat kidney. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841]

Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841]

Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded mouse brain. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.
HIST1H3A Antibody

Western Blot: HIST1H3A Antibody [NBP2-16841] -

Western Blot: HIST1H3A Antibody [NBP2-16841] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
HIST1H3A Antibody

Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16841] -

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
HIST1H3A Antibody

Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody [NBP2-16841] -

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody (NBP2-16841) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for HIST1H3A Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Alternate Names

H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Gene Symbol

H3C1

Additional HIST1H3A Products

Product Documents for HIST1H3A Antibody

Certificate of Analysis

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Product Specific Notices for HIST1H3A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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