HIST1H3A [ac Lys18] Antibody

Novus Biologicals | Catalog # NBP2-43535

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HIST1H3A Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys18 of human HIST1H3A. The exact sequence is proprietary.

Modification

ac Lys18

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIST1H3A [ac Lys18] Antibody

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.
Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.
Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.
Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.
Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535]

Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract:
A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr)
B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE

The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
HIST1H3A [ac Lys18] Antibody

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HIST1H3A [ac Lys18] Antibody

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Scale bar= 10um.
HIST1H3A [ac Lys18] Antibody

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HIST1H3A [ac Lys18] Antibody

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Scale bar= 10um.
HIST1H3A [ac Lys18] Antibody

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HIST1H3A [ac Lys18] Antibody

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
HIST1H3A [ac Lys18] Antibody

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
HIST1H3A [ac Lys18] Antibody

Dot Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Dotblot analysis of anti-Histone H3 (acetyl Lys18) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys18) antibody (NBP2-43535) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac
HIST1H3A [ac Lys18] Antibody

Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (NBP2-43535) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.
Blue: Hoechst 33342 staining.
HIST1H3A [ac Lys18] Antibody

Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody (NBP2-43535) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HIST1H3A [ac Lys18] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000
Application Notes
Dot Blot, Chip assay- Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIST1H3A

Alternate Names

H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Gene Symbol

H3C1

Additional HIST1H3A Products

Product Documents for HIST1H3A [ac Lys18] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HIST1H3A [ac Lys18] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for HIST1H3A [ac Lys18] Antibody

Customer Reviews for HIST1H3A [ac Lys18] Antibody

There are currently no reviews for this product. Be the first to review HIST1H3A [ac Lys18] Antibody and earn rewards!

Have you used HIST1H3A [ac Lys18] Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies