Histamine H2R Antibody

Novus Biologicals | Catalog # NB600-812

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Histamine H2 R Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QEEKPLKLQVWSGTE corresponding to C-Terminus according to NP_071640.1, NP_001124527.1.

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Histamine H2R Antibody

Western Blot: Histamine H2R Antibody [NB600-812]

Western Blot: Histamine H2R Antibody [NB600-812]

Western Blot: Histamine H2R Antibody [NB600-812] - Staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Histamine H2R Antibody [NB600-812]

Immunocytochemistry/ Immunofluorescence: Histamine H2R Antibody [NB600-812]

Immunocytochemistry/Immunofluorescence: Histamine H2R Antibody [NB600-812] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Histamine H2R Antibody [NB600-812]

Flow Cytometry: Histamine H2R Antibody [NB600-812]

Flow Cytometry: Histamine H2R Antibody [NB600-812] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Histamine H2R Antibody

Immunohistochemistry: Histamine H2R Antibody [NB600-812] -

Nuclear localization of H2R. (A.1) Confocal images of LECs, labeled for Prox-1 (green channel), H2R (red channel), nuclei (DAPI) and overlay of all three channels. (A.2) Inset of one cell zoomed, which indicates the localization of H2R, Prox-1 and DAPI. (B) IHC staining performed on the rat perilymphatic tissue. The tissue was stained with H2R antibody, showing its nuclear localization; the inset zoomed view shows the localization pattern. Images are representative of at least three independent experiments. Scale bar: 20 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35776777), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Histamine H2R Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/mL
Application Notes
Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to NP_001354640.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histamine H2 R

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Long Name

Histamine H2 Receptor

Alternate Names

Gastric Receptor 1, Histamine H2R, HRH2

Entrez Gene IDs

3274 (Human)

Gene Symbol

HRH2

UniProt

NP_071640.1 (Human)

Additional Histamine H2 R Products

Product Documents for Histamine H2R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Histamine H2R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histamine H2R Antibody

Customer Reviews for Histamine H2R Antibody

There are currently no reviews for this product. Be the first to review Histamine H2R Antibody and earn rewards!

Have you used Histamine H2R Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies