Histone Deacetylase 2/HDAC2 Antibody (9H4) - BSA Free

Novus Biologicals | Catalog # NBP3-26239

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H4 expressed in HEK293

Format

BSA Free
View all Histone Deacetylase 2/HDAC2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Histone Deacetylase 2/HDAC2 [UniProt Q92769]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (9H4) - BSA Free

Histone Deacetylase 2/HDAC2 Antibody (9H4)

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Histone Deacetylase 2/HDAC2 Antibody (9H4)

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Histone Deacetylase 2/HDAC2 Antibody (9H4)

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Positive Western Blot detected in: MCF-7 whole cell lysate, K562 whole cell lysate.
All lanes: Histone Deacetylase 2/HDAC2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 56, 52 kDa
Observed band size: 60 kDa
Histone Deacetylase 2/HDAC2 Antibody (9H4)

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] -

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Staining of Hela Cells with Histone Deacetylase 2/HDAC2 Antibody (9H4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Histone Deacetylase 2/HDAC2 Antibody (9H4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (9H4) - BSA Free

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (9H4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail