Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Novus Biologicals | Catalog # NBP3-16713
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]](https://resources.rndsystems.com/images/products/Histone-Deacetylase-8-HDAC8-Antibody-1O2Y5-Immunohistochemistry-Paraffin-NBP3-16713-img0002.jpg "Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1O2Y5 expressed in HEK293
Loading...
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 8/HDAC8 (Q9BY41). MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Rat brain using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]](https://resources.rndsystems.com/images/products/Histone-Deacetylase-8-HDAC8-Antibody-1O2Y5-Immunoprecipitation-NBP3-16713-img0008.jpg "Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]")
Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Analysis of 300ug extracts of HeLa cells using 3ug Histone Deacetylase 8/HDAC8 antibody (NBP3-16713). Western blot was performed from the immunoprecipitate using Histone Deacetylase 8/HDAC8 antibody (NBP3-16713) at a dilition of 1:1000.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] -
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Confocal imaging of HeLa cells using [KO Validated] Histone Deacetylase 8/HDAC8 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Applications for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:100 - 1:1000
Immunohistochemistry-Paraffin
1:100 - 1:1000
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 8/HDAC8
Alternate Names
HDAC8, HDACL1, RPD3
Gene Symbol
HDAC8
Additional Histone Deacetylase 8/HDAC8 Products
Product Documents for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
There are currently no reviews for this product. Be the first to review Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) and earn rewards!
Have you used Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...