Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00055869-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2F4

Format

Azide and BSA Free
View all Histone Deacetylase 8/HDAC8 Primary Antibodies »

Product Specifications

Immunogen

HDAC8 (AAH50433, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV

Reactivity Notes

Human. Other species not tested.

Specificity

histone deacetylase 8

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01]

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01]

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - HDAC8 monoclonal antibody (M01), clone 2F4 Analysis of HDAC8 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Analysis of monoclonal antibody to HDAC8 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01]

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01]

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Analysis of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M01), clone 2F4.Lane 1: HDAC8 transfected lysate(41.8 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Detection limit for recombinant GST tagged HDAC8 is approximately 0.3ng/ml as a capture antibody.

Applications for Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 8/HDAC8

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Alternate Names

HDAC8, HDACL1, RPD3

Entrez Gene IDs

55869 (Human)

Gene Symbol

HDAC8

OMIM

300269 (Human)

UniProt

AAH50433

Additional Histone Deacetylase 8/HDAC8 Products

Product Documents for Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Histone Deacetylase 8/HDAC8 Antibody (2F4) - Azide and BSA Free

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Protocols

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FAQs

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