Histone H2B type 1B [p Ser14] Antibody (RM238)
Novus Biologicals | Catalog # NBP2-61501
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Vertebrate
Applications
Multiplex Immunofluorescence, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM238 expressed in HEK293
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Product Specifications
Immunogen
A phospho-peptide corresponding to Histone H2B type 1B [p Ser14]
Modification
p Ser14
Specificity
This antibody reacts to Histone H2B type 1B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2B type 1B [p Ser14] Antibody (RM238)
![Histone H2B type 1B [p Ser14] Antibody (RM238)](https://resources.rndsystems.com/images/products/nbp2-61501_rabbit-monoclonal-histone-h2b-type-1b-p-ser14-antibody-rm238-immunocytochemistry-immunofluorescence-18920252338179.png "Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP2-61501] -")
Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP2-61501] -
Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP2-61501] - Immunocytochemistry of HeLa cells using Clone RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2B type 1B [p Ser14] Antibody (RM238)
Application
Recommended Usage
ELISA
0.2 ug/mL-1 ug/mL
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2B type 1B
Alternate Names
H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804
Gene Symbol
H2BC3
Additional Histone H2B type 1B Products
Product Documents for Histone H2B type 1B [p Ser14] Antibody (RM238)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H2B type 1B [p Ser14] Antibody (RM238)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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