Histone H3 Antibody - BSA Free

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• Histone H3 Antibody - BSA Free

  Name: Anonymous

  Application: Western Blot

  Sample Tested: 293 whole cell lysate

  Species: Human

  Verified Customer | Posted 01/26/2023

  first lane is 293T protein, second lane is kdm5c knockdown 293T protein

  

• Histone H3 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Paraffin

  Sample Tested: Pancreas tissue

  Species: Mouse

  Verified Customer | Posted 02/13/2022

  Mouse pancreas tissue

  Histone H3 antibody dilution 1:200

  

• Histone H3 Antibody

  Name: Anonymous

  Application: Immunofluorescence

  Sample Tested: human blood

  Species: Human

  Verified Customer | Posted 07/30/2021

  Immunofluorescence staining to detect histone H3 in human blood.

  

• Histone H3 Antibody

  Name: Adeline Williams

  Application: Western Blot

  Sample Tested: Ovary tissue, Midgut tissue and A549 Nuclear and cytoplasmic, Sample Amount: 30ug

  Species: Ae. aegypti

  Verified Customer | Posted 05/22/2021

  Western blot and Commassie-stained SDS/PAGE gels of Ae. aegypti ovary and midgut tissues where cytoplasmic and nuclear fractions have been isolated.

  To test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction (perhaps due to nuclear contamination in the cytoplasmic fraction). anti-beta-tubulin did not react well in midgut fractions. For your reference, protein ladder = pageruler plus prestained ThermoFisher

  

• Histone H3 Antibody

  Name: Anonymous

  Application: Western Blot

  Sample Tested: Breast cancer cells

  Species: Human

  Verified Customer | Posted 05/18/2020

  MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed.

  

• Histone H3 Antibody

  Name: Anonymous

  Application: Western Blot

  Sample Tested: Human breast cancer cell lines

  Species: Human

  Verified Customer | Posted 10/22/2018

  Lysates from human breast cancer cells were separated into nuclear and cytosolic proteins and antibodies against alpha-tubulin and Histone H3 were used as markers.

  

• Histone H3 Antibody

  Name: Anonymous

  Application: Western Blot

  Sample Tested: MDA-MB-231 cells (cytoplasmic and nuclear fractions)

  Species: Human

  Verified Customer | Posted 12/27/2016

  Immunoblots of cytoplasmic and nuclear fractions of MDA-MB-231 cells. We used Novus anti-Histone H3 antibody NB500-171 to demonstrate purity of the fractions.

  

• Name: Bryan Tinsley

  Application: Western Blot

  Sample Tested:

  Species: Human

  Verified Customer | Posted 06/13/2014

  Western blot analysis of extracts from HeLa, COS, C6 and K562 cells using Histone H3 antibody (NB500-171, 1:100).

  

• Name: Anonymous

  Application: Western Blot

  Sample Tested: Whole cell extract from MCF-7 and HCT-116 cells

  Species: Human

  Verified Customer | Posted 06/10/2014

  Total histone H3 levels in MCF-7 and HCT-116 cells

  

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