Histone H3.3B Antibody (2D7-H1) - Azide and BSA Free
Novus Biologicals | Catalog # H00003021-M01
![Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]](https://resources.rndsystems.com/images/products/Histone-H3-3B-Antibody-2D7-H1-Western-Blot-H00003021-M01-img0009.jpg "Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine, Drosophila, Xenopus
Cited:
Mouse, Frog - Xenopus (African Clawed Frog)
Applications
Validated:
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Knockout Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2D7-H1
Format
Azide and BSA Free
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Product Specifications
Immunogen
H3F3B (AAH17558, 1 a.a. ~ 136 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 23903189). Xenopus reactivity reported in scientific literature (PMID: 23102146). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
H3F3B - H3 histone, family 3B (H3.3B)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Histone H3.3B Antibody (2D7-H1) - Azide and BSA Free
Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]
Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.Lane 1: H3F3B transfected lysate(15 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]](https://resources.rndsystems.com/images/products/Histone-H3-3B-Antibody-2D7-H1-Immunocytochemistry-Immunofluorescence-H00003021-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]")
Immunocytochemistry/ Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]
Immunocytochemistry/Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on HeLa cell. Antibody concentration 10 ug/ml.![Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]](https://resources.rndsystems.com/images/products/Histone-H3-3B-Antibody-2D7-H1-Immunohistochemistry-Paraffin-H00003021-M01-img0007.jpg "Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]")
Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]
Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.![ELISA: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]](https://resources.rndsystems.com/images/products/Histone-H3-3B-Antibody-2D7-H1-ELISA-H00003021-M01-img0010.jpg "ELISA: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]")
ELISA: Histone H3.3B Antibody (2D7-H1) [H00003021-M01]
ELISA: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Detection limit for recombinant GST tagged H3F3B is 1 ng/ml as a capture antibody.Applications for Histone H3.3B Antibody (2D7-H1) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, IF and ELISA. Knock Out Validation was reported in scientific literature (PMID: 26159997).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Histone H3.3B
Alternate Names
H3 histone, family 3A, H3 histone, family 3B (H3.3B), H3.3BH3.3A, H3F3, H3F3A, histone H3.3
Entrez Gene IDs
3021 (Human)
Gene Symbol
H3-3B
OMIM
601058 (Human)
UniProt
Additional Histone H3.3B Products
Product Documents for Histone H3.3B Antibody (2D7-H1) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Histone H3.3B Antibody (2D7-H1) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Histone H3.3B Antibody (2D7-H1) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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