Histone H4 [ac Lys8] Antibody (RM201)

Name: Histone H4 [ac Lys8] Antibody (RM201)
Brand: Novus Biologicals
SKU: NBP2-61558
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61558

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Vertebrate

Applications

Multiplex Immunofluorescence, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM201 expressed in HEK293

View all Histone H4 Primary Antibodies »

Product Specifications

Immunogen

An acetyl-peptide corresponding to the Histone H4 [ac Lys8]

Modification

ac Lys8

Specificity

This antibody reacts to Histone H4 acetylated at Lysine 8. No cross reactivity with other acetylated Lysines in Histone H4.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [ac Lys8] Antibody (RM201)

Western Blot: Histone H4 [ac Lys8] Antibody (RM201) [NBP2-61558] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4. Using NBP2-61558, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP2-61558] -

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody (RM201) - Azide and BSA Free [NBP2-61558] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM201 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Applications for Histone H4 [ac Lys8] Antibody (RM201)

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1ug-5 ug

ELISA

0.2 ug/mL - 1 ug/mL

Immunocytochemistry/ Immunofluorescence

0.5 ug/ml - 2 ug/ml

Western Blot

0.5 ug/ml-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys8] Antibody (RM201)

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Certificate of Analysis

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Product Specific Notices for Histone H4 [ac Lys8] Antibody (RM201)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Notch Pathway

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